VPS39 Antibody

Datasheet

Code	CSB-PA836235LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue All lanes: VPS39 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 102, 101 kDa Observed band size: 102 kDa Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA836235LA01HU at dilution of 1:100 Immunofluorescent analysis of A549 cells using CSB-PA836235LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Western Blot Positive WB detected in: SH-SY5Y whole cell lysate All lanes: VPS39 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 102, 101 kDa Observed band size: 102 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VPS39 Polyclonal antibody

Uniprot No.

Q96JC1

Target Names

VPS39

Alternative Names

A230065P22Rik antibody; AW743070 antibody; FLJ21681 antibody; FLJ46546 antibody; hVam6p antibody; KIAA0770 antibody; RP23-348L16.12 antibody; TLP antibody; TRAP1 like protein antibody; Vacuolar protein sorting 39 antibody; Vacuolar protein sorting 39 homolog antibody; VAM6 antibody; Vam6/Vps39 like antibody; Vam6/Vps39 like protein antibody; Vam6/Vps39-like protein antibody; VPS 39 antibody; Vps39 antibody; VPS39_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Vam6/Vps39-like protein (263-490AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The VPS39 Antibody (Product code: CSB-PA836235LA01HU) is Non-conjugated. For VPS39 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836235LB01HU	VPS39 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836235LC01HU	VPS39 Antibody, FITC conjugated
Biotin	CSB-PA836235LD01HU	VPS39 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The recombinant peptide corresponding to amino acids 263-490 of the human VPS39 is used as the immunogen to immunize the rabbit to yield the anti-VPS39 antibody. The VPS39 polyclonal antibody exists as an unconjugated IgG isoform. Its purity is 95%⁺ using protein G purified. It reacts with human and mouse VPS39. This VPS39 antibody has been validated for use in ELISA, WB, IF, and IHC analyses.

VPS39 mainly regulates endosomal membrane trafficking and fusion. It is a subunit of the homotypic fusion and protein sorting (HOPS) complex, which is involved in the tethering and fusion of endosomes and lysosomes. It is also involved in the regulation of lysosomal biogenesis, autophagy, and signaling pathways. Mutations in the VPS39 gene have been linked to various diseases such as Hermansky-Pudlak syndrome.

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Target Background

Function

Regulator of TGF-beta/activin signaling, inhibiting SMAD3- and activating SMAD2-dependent transcription. Acts by interfering with SMAD3/SMAD4 complex formation, this would lead to inhibition of SMAD3-dependent transcription and relieve SMAD3 inhibition of SMAD2-dependent promoters, thus increasing SMAD2-dependent transcription. Does not affect TGF-beta-induced SMAD2 or SMAD3 phosphorylation, nor SMAD2/SMAD4 complex formation.; Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes. Required for fusion of endosomes and autophagosomes with lysosomes.

Gene References into Functions

TLP expression contributes to hypertrophic scar formation and contraction. PMID: 25655281
TLP likely acts as a molecular modulator capable of altering the balance of Smad3- and Smad2-dependent signaling through regulation of phosphorylation, thus facilitating collagen synthesis in fibroblasts. PMID: 23418473
Vps39 knockdown impairs late endosome fusion and fusion between late endosomes and lysosomes. PMID: 23167963
Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus PMID: 21454559
although only TBC1D15/Rab7-GAP altered Rab7-GTP levels, both Rab7-GAP and mVps39 regulate lysosomal morphology and play a role in maintaining growth factor dependence PMID: 20363736
propose that TLP might regulate the balance of Smad2 and Smad3 signaling by localizing Smad4 intracellularly, thus contributing to cellular specificity of TGF-beta transcriptional responses in both normal and pathophysiology PMID: 12941698

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Subcellular Location

Cytoplasm. Lysosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein.;

Protein Families

VAM6/VPS39 family

Tissue Specificity

Widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen.

Database Links

HGNC: 20593

OMIM: 612188

KEGG: hsa:23339

STRING: 9606.ENSP00000326534

UniGene: Hs.88025

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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