Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VPS4A Polyclonal antibody

Uniprot No.

Q9UN37

Target Names

VPS4A

Alternative Names

hVPS4 antibody; Protein SKD2 antibody; SKD1 antibody; SKD1 homolog antibody; SKD1A antibody; SKD2 antibody; Vacuolar protein sorting 4 homolog A antibody; Vacuolar protein sorting 4, yeast, homolog of, A antibody; Vacuolar protein sorting factor 4A antibody; Vacuolar protein sorting-associated protein 4A antibody; vacuolar sorting protein 4 antibody; VPS4 antibody; VPS4-1 antibody; Vps4a antibody; VPS4A_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Vacuolar protein sorting-associated protein 4A protein (1-120AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Generating the polyclonal antibody against the recombinant human VPS4A protein (1-120aa) involves immunizing a rabbit with the target antigen, collecting the resulting rabbit serum, and purifying the antibody-containing serum through antigen affinity chromatography.

The purified VPS4A polyclonal antibody has been characterized by ELISA, WB, and IHC to confirm their specificity and sensitivity to VPS4A. This antibody can react with human, mouse, and rat VPS4A proteins. VPS4A mainly catalyzes the disassembly of ESCRT complexes, which are involved in the sorting and trafficking of membrane proteins in the endocytic pathway. It is also implicated in cytokinesis and virus budding.

Target Background

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.; (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

Gene References into Functions

These results demonstrate that the ESCRT machinery, and in particular VPS4, plays a critical role in the early stages of PV infection. PMID: 28349933
Continuous ESCRT-III remodelling by dynamic subunit turnover is regulated by Vps4 to mediate membrane remodelling during cytokinesis. PMID: 28604678
associations were noted between the striatal node, hyperactivity, and the vacuolar protein sorting-associated protein 4A (VPS4A) gene in humans, and the causal role of Vps4 for hyperactivity was validated in Drosophila PMID: 27001827
These results point to the existence of previously undetected biological functions of the FTase alpha subunit that includes control of intracellular membrane trafficking. PMID: 26551458
these experiments suggest that miR-16 and VPS4a expression are altered in end-stage heart failure and in response to unloading with a left ventricular assist device PMID: 25033200
Ca2+-dependent accumulation of ESCRT III-Vps4 complex following large focal injury to the cell membrane and identify the role of ALG-2 as the initiator of sequential ESCRT III-Vps4A/B complex assembly. PMID: 25534348
Vps4A may function as a tumor suppressor, which utilizes exosomes as mediators to regulate the secretion and uptake of miRNAs in hepatoma cells; these observations provide new insights into the development of HCC. PMID: 25503676
Vps4 stimulatory element of the cofactor Vta1 contacts the ATPase Vps4 alpha7 and alpha9 subunits to stimulate ATP hydrolysis. PMID: 25164817
Propose that the abscission checkpoint is mediated by ANCHR and CHMP4C through retention of VPS4 at the midbody ring. PMID: 24814515
VPS4 is required for endosomal transport of LDL cholesterol in HeLa cells. PMID: 23009658
silencing CHMP6 and VPS4A also blocked epidermal growth factor receptor (EGFR) recycling PMID: 22231449
VPS4, a master regulator of MVB sorting, may serve as a determinant of lysosomal targeting or extracellular secretion of alphaSYN and thereby contribute to the intercellular propagation of Lewy pathology in PD PMID: 22216284
These results indicate that VPS4A has a direct role in membrane scission leading to HIV-1 release. PMID: 21394086
Findings reveal new ways in which VPS4 activity is regulated and specifically directed to ESCRT-III polymers. PMID: 20805225
It was found that neither siRNA knockdown of VPS4A and VPS4B expression nor the use of cell lines that inducibly express VPS4A or VPS4B dominant negative mutants, inhibited influenza virus budding. PMID: 20621136
ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission, thereby helping ensure the ordered progression through the different stages of cell division. PMID: 20616062
Vps4 and the ESCRT-III complex are required for the release of infectious hepatitis C virus particles. PMID: 19828764
SKD1-dependent endosomal membrane trafficking is required for formation of autolysosomes. PMID: 11937716
Data suggested that hVPS4 is involved in the release of components of the bilayered coat from the endosomal membrane. PMID: 15075231
hSnf7-1 binds to itself, membranes, and the AAA+ ATPase SKD1 PMID: 15632132
The solution structure of the N-terminal VPS4A microtubule interacting and transport (MIT) domain was reported. PMID: 16174732
in cells expressing dominant negative VPS4a, doxorubicin accumulates in VPS4a-positive vesicles and drug sequestration is inhibited PMID: 16841193
Upon coexpression of mutated CHMP3, CHMP4B, or CHMP4C forms, as well as of ATPase-defective Vps4A or Vps4B mutants, HBV assembly and egress were potently blocked. PMID: 17553870
Inhibits Vesicular Stomatitis Virus budding but does not affect Semliki Forest Virus budding. PMID: 17913808
studies reveal how the VPS4 ATPases recognize their CHMP substrates to facilitate the membrane fission events required for the release of viruses, endosomal vesicles and daughter cells PMID: 17928862
Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. PMID: 18621683
Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. PMID: 19524996
Both Vps4A and CHMP1A localized in the vicinity of viral cytoplasmic assembly compartments, sites of viral maturation that develop in Cytomegalovirus-infected cells. Thus, ESCRT machinery is involved in the final steps of HCMV replication. PMID: 19640981

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Subcellular Location

Late endosome membrane; Peripheral membrane protein. Midbody.

Protein Families

AAA ATPase family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 13488

OMIM: 609982

KEGG: hsa:27183

STRING: 9606.ENSP00000254950

UniGene: Hs.128420

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Code	CSB-PA891974DSR2HU
Size	US$166
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Image	Western blot All lanes: VPS4Aantibody at 4.28μg/ml Lane 1: Mouse kidney tissue Lane 2: Rat gonadal tissue Lane 3: Mouse brain tissue Lane 4: U87 whole cell lysate Lane 5: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA891974DSR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA891974DSR2HU at dilution of 1:100
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