Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VPS4B Polyclonal antibody

Uniprot No.

O75351

Target Names

VPS4B

Alternative Names

8030489C12Rik antibody; Cell migration inducing 1 antibody; Cell migration-inducing gene 1 protein antibody; MGC116271 antibody; MIG1 antibody; Protein SKD1 antibody; Skd1 antibody; SKD1B antibody; Suppressor of K(+) transport growth defect 1 antibody; Suppressor of K+ transport defect 1 antibody; Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody; Vacuolar protein sorting 4 homolog B antibody; Vacuolar protein sorting 4b antibody; Vacuolar protein sorting-associated protein 4B antibody; Vacuolar protein sorting-associating protein 4B antibody; VPS4 2 antibody; VPS42 antibody; Vps4b antibody; VPS4B_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Vacuolar protein sorting-associated protein 4B protein (1-117AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Biotin

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.; (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).

Gene References into Functions

VPS4B might facilitate chondrocyte apoptosis in Osteoarthritis via p38 MAPK signaling pathway. PMID: 28744712
Knockdown of vps4b in zebrafish recapitulated the reduction of tooth size. PMID: 27247351
Our findings support a role for VPS4B in MM cell proliferation, adhesion, and drug resistance, and pave the way for a novel therapeutic approach targeting this molecule. PMID: 25804841
Lack of ALG-2, ALIX or Vps4B each prevents shedding, and repair of the injured cell membrane PMID: 25534348
Crystal structures of three molecular complexes reveal that IST1 binds to the MIT domains of VPS4 and LIP5. PMID: 25657007
ESCRT-III protein CHMP5 inhibits LIP5-mediated VPS4 activation by inducing a moderate conformational change within LIP5. PMID: 25637630
High Vacuolar protein sorting 4B results in more apoptosis of intestinal epithelial cells via p38 MAPK in Crohn's disease. PMID: 25533544
Taken together, it was concluded that VPS4B and its DN mutant VPS4B-K180Q have anti-HBV effect in vivo, which helps develop molecular therapeutic strategies for HBV infection. PMID: 22684550
Protein kinase CK2 alpha is involved in the phosphorylation of the ESCRT-III subunits CHMP3 and CHMP2B, as well as of VPS4B/SKD1, an ATPase that mediates ESCRT-III disassembly. PMID: 24440309
VPS4B may promote the progression of Carcinoma, Non-Small-Cell Lung (NSCLC) and be a biotarget for NSCLCs therapy. PMID: 23737133
An inverse correlation between VPS4B expression and EGFR abundance is observed in breast tumors, and high-grade or recurrent breast carcinomas exhibit lower VPS4B expression. PMID: 22252323
It was found that neither siRNA knockdown of VPS4A and VPS4B expression nor the use of cell lines that inducibly express VPS4A or VPS4B dominant negative mutants, inhibited influenza virus budding. PMID: 20621136
ESCRT-III/VPS4 proteins function at centrosomes to help regulate their maintenance or proliferation and then at midbodies during abscission, thereby helping ensure the ordered progression through the different stages of cell division. PMID: 20616062
The expression and transport of ALG-2 in association with TSG101 and Vps4B are reported. PMID: 16004603
We propose that the MIT domain of Vps4b serves as a putative adaptor domain for the ESCRT-III complex involved in endosomal trafficking. PMID: 16018968
The crystal structure of monomeric apo human VPS4B was reported. PMID: 16193069
Four proteins (TSG101,Hrs,Aip1/Alix, and Vps4B) of the ESCRT (endosomal sorting complex required for transport) machinery were localized in T cells and macrophages by quantitative electron microscpy. PMID: 17014699
Upon coexpression of mutated CHMP3, CHMP4B, or CHMP4C forms, as well as of ATPase-defective Vps4A or Vps4B mutants, HBV assembly and egress were potently blocked. PMID: 17553870
Here, we report that dominant negative forms of Vps4A, Vps4B, and AIP1 inhibit HTLV-1 budding. PMID: 17601348
studies reveal how the VPS4 ATPases recognize their CHMP substrates to facilitate the membrane fission events required for the release of viruses, endosomal vesicles and daughter cells PMID: 17928862
ATP hydrolysis would eliminate this interaction and subsequent nucleotide release causes the domains to rotate, which together lead to the disassembly of the SKD1 oligomer PMID: 18796009
Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. PMID: 19524996