WAPL Antibody

Datasheet

Code	CSB-PA772025LA01HU
Size	US$166
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Image	IHC image of CSB-PA772025LA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA772025LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Hela whole cell lysate, K562 whole cell lysate All lanes: WAPL antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 133, 138, 143 kDa Observed band size: 133 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WAPL Polyclonal antibody

Uniprot No.

Q7Z5K2

Target Names

WAPL

Alternative Names

FOE antibody; Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody; Friend of EBNA2 protein antibody; KIAA0261 antibody; OTTHUMP00000020004 antibody; WAPAL antibody; WAPL antibody; WAPL_HUMAN antibody; Wings apart like homolog antibody; Wings apart like homolog (Drosophila) antibody; Wings apart-like protein homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Wings apart-like protein homolog protein (1037-1190AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WAPL Antibody (Product code: CSB-PA772025LA01HU) is Non-conjugated. For WAPL Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA772025LB01HU	WAPL Antibody, HRP conjugated	ELISA
FITC	CSB-PA772025LC01HU	WAPL Antibody, FITC conjugated
Biotin	CSB-PA772025LD01HU	WAPL Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

Gene References into Functions

Study shows that chromatin loop size can be increased and that the duration with which cohesin embraces DNA determines the degree to which loops are enlarged. Cohesin's DNA release factor WAPL restricts this loop extension and also prevents looping between incorrectly oriented CTCF sites. PMID: 28475897
Rs7083506 and rs11202058 polymorphisms of hWAPL and their haplotype T-A were associated with cervical cancer. PMID: 26722608
WAPL-depleted cells undergo anaphase with segregation errors, resulting in micronuclei and DNA damage. Stable WAPL depletion arrests cells in a p53-dependent manner but causes p53-deficient cells to become highly aneuploid. PMID: 24055153
Wapl-C interacts with other cohesin subunits and possibly unknown effectors to trigger cohesin release from chromatin PMID: 23776203
Depletion of Wapl, a negative cohesin regulator, rescues sister chromatid homologous recombination defects of Mms21-deficient or Scc1 mutant-expressing cells PMID: 22751501
FOE encodes a protein of 1227 amino acids with a functional bipartite nuclear localization signal, an arginine-rich motif, a putative nuclear export signal as well as with three highly acidic regions and a predicted coiled-coil domain. PMID: 15383329
Wapl is a new regulator of sister chromatid resolution and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits PMID: 17112726
Wapl is required to unlock cohesin from a particular state in which it is stably bound to chromatin. PMID: 17113138
our study suggests that unscheduled overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. PMID: 17382297
isolated a large number of additional alternatively spliced WAPL variants from various cervical epithelia. Each variant consists of a variable 5'-terminal region and the conserved remainder. PMID: 18662753

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Subcellular Location

[Isoform 2]: Nucleus.; Nucleus. Chromosome. Cytoplasm. Note=Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.

Protein Families

WAPL family

Tissue Specificity

Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.

Database Links

HGNC: 23293

OMIM: 610754

KEGG: hsa:23063

STRING: 9606.ENSP00000298767

UniGene: Hs.203099

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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