WDR73 Antibody

Datasheet
Code CSB-PA744047LA01HU
Size US$299
Uniprot No. Q6P4I2
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate
    All lanes: WDR73 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • IHC image of CSB-PA744047LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of SH-SY5Y cells with CSB-PA744047LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human WD repeat-containing protein 73 protein (163-366AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Relevance May play a role in the regulation of microtubule organization and dynamics (PubMed:25466283).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias WD repeat-containing protein 73, WDR73
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names WDR73
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function May play a role in the regulation of microtubule organization and dynamics
Involvement in disease Galloway-Mowat syndrome 1 (GAMOS1)
Subcellular Location Cytoplasm, cytosol, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, spindle pole, Cleavage furrow
Protein Families WD repeat WDR73 family
Tissue Specificity Expressed in kidney and brain. In the kidney, expressed in glomeruli, most probably in podocytes, and in tubules (at protein level). In the brain, expressed in the cerebellum, with high levels in Purkinje cells and their projecting axons, in the deep cere
Database Links

HGNC: 25928

OMIM: 251300

KEGG: hsa:84942

STRING: 9606.ENSP00000387982

UniGene: Hs.745027

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