WDR73 Antibody

Datasheet

Code	CSB-PA744047LA01HU
Size	US$299
Image	Western Blot Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate All lanes: WDR73 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa IHC image of CSB-PA744047LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA744047LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Description

The antigen affinity purified Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody is generated in rabbits using Recombinant Human WD repeat-containing protein 73 (163-366aa) as the immunogen. This product is tested for use in WB, ELISA, IHC, and IF.
The target, WDR73, belongs to one of the largest protein families, the WD40-repeat proteins. It may play a significant role in the functions of microtubule and spindle poles during mitotic cell division. Aberrations in its actions are linked to Galloway-Mowat Syndrome. Also, the reduced expression of the WDR73 gene has been linked to irregular nuclear structure and brain structure aberrations.
The Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody reacts with Human WDR73. Thus, it is used to study the role of WDR73 in Galloway-Mowat Syndrome and some other brain defects.

Full Product Name

Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody

Uniprot No.

Q6P4I2

Target Names

WDR73

Alternative Names

WDR73 antibody; HSPC264WD repeat-containing protein 73 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human WD repeat-containing protein 73 protein (163-366AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WDR73 Antibody (Product code: CSB-PA744047LA01HU) is Non-conjugated. For WDR73 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA744047LB01HU	WDR73 Antibody, HRP conjugated	ELISA
FITC	CSB-PA744047LC01HU	WDR73 Antibody, FITC conjugated
Biotin	CSB-PA744047LD01HU	WDR73 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function	May play a role in the regulation of microtubule organization and dynamics.
Gene References into Functions	We expanded the clinical phenotype of GMS with WDR73 gene defect to include retinal dysfunction with missense mutation and developmental dysplasia of the hip. PMID: 29929488 WDR73 as a candidate gene of severe intellectual disability and cerebellar hypoplasia. PMID: 27983999 We document postnatal onset of CA, a retinopathy, basal ganglia degeneration, and short stature as novel features of WDR73-related disease, and define WDR73-related disease as a new entity of infantile neurodegeneration. PMID: 26123727 Nonsense mutation in the WDR73 gene is associated with Galloway-Mowat syndrome PMID: 25873735 WDR73 interacts with mitotic microtubules to regulate cell cycle progression, proliferation and survival in brain PMID: 26070982 WDR73 plays a crucial role in the maintenance of cell architecture and cell survival. PMID: 25466283 Show More Hide All
Involvement in disease	Galloway-Mowat syndrome 1 (GAMOS1)
Subcellular Location	Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow.
Protein Families	WD repeat WDR73 family
Tissue Specificity	Expressed in kidney and brain. In the kidney, expressed in glomeruli, most probably in podocytes, and in tubules (at protein level). In the brain, expressed in the cerebellum, with high levels in Purkinje cells and their projecting axons, in the deep cere
Database Links	HGNC: 25928 OMIM: 251300 KEGG: hsa:84942 STRING: 9606.ENSP00000387982 UniGene: Hs.745027

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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WDR73 Antibody

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