WDR73 Antibody

Code CSB-PA744047LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, U87 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, THP-1 whole cell lysate
    All lanes: WDR73 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • IHC image of CSB-PA744047LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of SH-SY5Y cells with CSB-PA744047LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Description

The antigen affinity purified Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody is generated in rabbits using Recombinant Human WD repeat-containing protein 73 (163-366aa) as the immunogen. This product is tested for use in WB, ELISA, IHC, and IF.
The target, WDR73, belongs to one of the largest protein families, the WD40-repeat proteins. It may play a significant role in the functions of microtubule and spindle poles during mitotic cell division. Aberrations in its actions are linked to Galloway-Mowat Syndrome. Also, the reduced expression of the WDR73 gene has been linked to irregular nuclear structure and brain structure aberrations.
The Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody reacts with Human WDR73. Thus, it is used to study the role of WDR73 in Galloway-Mowat Syndrome and some other brain defects.

Full Product Name Rabbit anti-Homo sapiens (Human) WDR73 Polyclonal antibody
Uniprot No. Q6P4I2
Target Names WDR73
Alternative Names WDR73 antibody; HSPC264WD repeat-containing protein 73 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human WD repeat-containing protein 73 protein (163-366AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function May play a role in the regulation of microtubule organization and dynamics
Gene References into Functions
  1. We expanded the clinical phenotype of GMS with WDR73 gene defect to include retinal dysfunction with missense mutation and developmental dysplasia of the hip. PMID: 29929488
  2. WDR73 as a candidate gene of severe intellectual disability and cerebellar hypoplasia. PMID: 27983999
  3. We document postnatal onset of CA, a retinopathy, basal ganglia degeneration, and short stature as novel features of WDR73-related disease, and define WDR73-related disease as a new entity of infantile neurodegeneration. PMID: 26123727
  4. Nonsense mutation in the WDR73 gene is associated with Galloway-Mowat syndrome PMID: 25873735
  5. WDR73 interacts with mitotic microtubules to regulate cell cycle progression, proliferation and survival in brain PMID: 26070982
  6. WDR73 plays a crucial role in the maintenance of cell architecture and cell survival. PMID: 25466283

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Involvement in disease Galloway-Mowat syndrome 1 (GAMOS1)
Subcellular Location Cytoplasm, cytosol, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, spindle pole, Cleavage furrow
Protein Families WD repeat WDR73 family
Tissue Specificity Expressed in kidney and brain. In the kidney, expressed in glomeruli, most probably in podocytes, and in tubules (at protein level). In the brain, expressed in the cerebellum, with high levels in Purkinje cells and their projecting axons, in the deep cere
Database Links

HGNC: 25928

OMIM: 251300

KEGG: hsa:84942

STRING: 9606.ENSP00000387982

UniGene: Hs.745027

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