Product Details

Uniprot No.

Target Names

WIPF1

Alternative Names

WIPF1 antibody; WASPIP antibody; WIP antibody; WAS/WASL-interacting protein family member 1 antibody; Protein PRPL-2 antibody; Wiskott-Aldrich syndrome protein-interacting protein antibody; WASP-interacting protein antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from the C-terminal region of Human WIP.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:5000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Plays a role in the reorganization of the actin cytoskeleton. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation. Plays a role in the formation of cell ruffles. Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.

Gene References into Functions

WIP deficiency should be considered as a cause for autosomal-recessive immunodeficiency PMID: 27742395
the present study identifies the WIPF1 gene as having novel oncogenic functions and playing an important role in the invasiveness and aggressiveness of thyroid cancer when aberrantly up-regulated by the BRAF V600E/MAPK pathway through its promoter demethylation. PMID: 27863429
WIP controls tumor growth by boosting signals that stabilize the YAP/TAZ complex via a mechanism mediated by the endocytic/endosomal system. PMID: 27851961
Results suggest that local invasiveness of ameloblastoma is dependent upon the migratory potential of its tumour cells as defined by their distribution of cortactin, N-WASP and WIP in correlation with F-actin cytoskeletal dynamics. PMID: 26752341
establish a new cancer stem cell signalling pathway downstream of mtp53 in which AKT2 regulates WIP and controls YAP/TAZ stability. PMID: 28166194
Knocking down WIP expression in A549 cells significantly reduced RhoA levels and WIP was found to interact with RhoA suggesting that WIP might be executing its function by regulating RhoA. PMID: 27939884
Study provides evidence that WIP and WIRE contribute to breast cancer cell invasiveness through coordinated roles. WIP seems necessary for the assembly of invasive protrusions, whereas WIRE regulates their maturation, which leads to matrix degradation. PMID: 27009365
WIP was shown to interact with various binding partners, including the signaling proteins Nck, CrkL and cortactin. PMID: 22837718
These findings reveal WIP as a previously unreported regulator of neuronal maturation and synaptic activity PMID: 21810783
These findings indicate that WIP deficiency should be suspected in patients with features of WAS in whom WAS sequence and mRNA levels are normal. PMID: 22231303
show that the N-WASP EVH1 domain specifically binds a 25 residue motif from the WASP Interacting Protein (WIP) PMID: 12437929
Only in the presence of WASP-interacting protein (WIP) can human WASP suppress the growth defect of Saccharomyces cerevisiae las17Delta strain. PMID: 16488394
A 1.3 mD multiprotein complex consisting of WASp-interacting protein (WIP), Wiskott-Aldrich syndrome protein (WASp), actin, and myosin IIA formigg during NK cell activation was identified with a role in complex formation and NK cell activity regulation. PMID: 16606694
WASP-interacting protein stabilizes Wiskott-Aldrich syndrome protein(WASP) and suggest that it may also be important for its function PMID: 17213309
In this review, in addition to its WASP-independent functions, the role of WIP is to regulate the activity and stability of WASP and shuttle WASP to areas of actin assembly. PMID: 17949983
WIP is involved in lytic granule transport and is essential for regulation of Natural killer cell cytotoxic function PMID: 18258743
Groups of colorectal cancer, breast cancer, and glioma patients with low expression of the WIPF1 co-expression module generally had a favorable prognosis. PMID: 19399471

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Involvement in disease

Wiskott-Aldrich syndrome 2 (WAS2)

Subcellular Location

Cytoplasmic vesicle. Cytoplasm, cytoskeleton. Cell projection, ruffle.

Protein Families

Verprolin family

Tissue Specificity

Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.

Database Links

HGNC: 12736

OMIM: 602357

KEGG: hsa:7456

STRING: 9606.ENSP00000352802

UniGene: Hs.128067