WIPI2 Antibody

Datasheet

Code	CSB-PA531726
Size	US$119
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Image	Immunohistochemical analysis of paraffin-embedded Human Skeletal Muslce Tissue using WIPI2 Rabbit pAb diluted at 1:200 Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using WIPI2 Rabbit pAb diluted at 1:200
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9Y4P8

Target Names

WIPI2

Alternative Names

ATG18B antibody; Atg21 antibody; CGI 50 antibody; DKFZp434J154 antibody; DKFZp686P02188 antibody; FLJ12979 antibody; FLJ14217 antibody; FLJ42984 antibody; WD repeat domain phosphoinositide-interacting protein 2 antibody; WD repeat domain; phosphoinositide interacting 2 antibody; WD40 repeat protein interacting with phosphoinositides 2 antibody; WIPI 2 antibody; WIPI-2 antibody; WIPI2 antibody; WIPI2_HUMAN antibody; WIPI49 like protein 2 antibody; WIPI49-like protein 2 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant Protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Purification Method

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
ELISA	1:10000

Protocols

Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.; Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.

Gene References into Functions

Data suggest that, in patients with diabetic kidney disease, urinary excretion of mRNAs for MAP1LC3A, WIPI2, and RB1CC1 is down-regulated as compared to healthy control subjects; these transcripts may serve as urinary autophagy biomarkers. (MAP1LC3A = microtubule associated protein 1 light chain 3; WIPI2 = WD repeat domain phosphoinositide-interacting protein 2; RB1CC1 = RB1 inducible coiled-coil 1) PMID: 28760651
Here the authors show that recruitment of WIPI2, itself essential for anti-bacterial autophagy, is dependent on the localization of catalytically active TBK1 to the vicinity of cytosolic bacteria. PMID: 27370208
The specific autophagosomal localization of both WIPI1 and WIPI2 (refered to as WIPI puncta) has been employed to assess autophagy using fluorescence microscopy methods, such as confocal and live-cell video microscopy PMID: 25462558
Data suggest WIPI1/WIPI2 co-localize with microtubule-associated light chain 3 and autophagy related proteins 2/14L, participate in biogenesis of phagosomes, autophagy, and mobilization of lipids to/from intracellular droplets. [review-like article] PMID: 25233424
WIPI-1 and WIPI-2 are functionally required in mediating the PI3P signal at the onset of autophagy in NB4 cells. PMID: 24991767
Data suggest that WIPI2b directly interacts with dimer of ATG16L1 (autophagy related 16-like 1) and this interaction is linked to production of phosphatidylinositol 3-phosphate in endoplasmic reticulum triggered by autophagosome formation. [REVIEW] PMID: 25233411
WIPI2b binds the membrane surrounding Salmonella and recruits the Atg12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella. PMID: 24954904
A role for WIPI2 in the progression of omegasomes into autophagosomes, is reported. PMID: 20505359
Freeze-fracture replica immunolabelling reveals WD-repeat protein interacting with phosphoinositides 1 and 2 (WIPI-1 and WIPI-2) as membrane components of autophagosomes and the plasma membrane (PM). PMID: 21564513
WIPI2 is a phosphatidylinsitol-3-phosphate binding protein required for starvation induced autophagy. PMID: 20505359

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Subcellular Location

Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

WD repeat SVP1 family

Tissue Specificity

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

Database Links

HGNC: 32225

OMIM: 609225

KEGG: hsa:26100

STRING: 9606.ENSP00000288828

UniGene: Hs.122363

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Autophagy Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Autophagy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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