ZKSCAN3 Antibody

Datasheet

Code	CSB-PA861122LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate, HL-60 whole cell lysate All lanes: ZKSCAN3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 44 kDa Observed band size: 44 kDa IHC image of CSB-PA861122LA01HU diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cells with CSB-PA861122LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZKSCAN3 Polyclonal antibody

Uniprot No.

Q9BRR0

Target Names

ZKSCAN3

Alternative Names

dJ874C20.1 antibody; mszf35 antibody; SCAN-KRAB-zinc finger protein antibody; Skz1 antibody; Zf47 antibody; Zfp-47 antibody; ZFP306 antibody; Zfp307 antibody; Zfp47 antibody; Zinc finger and SCAN domain containing protein 13 antibody; Zinc finger and SCAN domain-containing protein 13 antibody; Zinc finger protein 306 antibody; Zinc finger protein 306; isoform CRA_a antibody; Zinc finger protein 307 antibody; Zinc finger protein 309 antibody; Zinc finger protein 47 homolog antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein zfp47 antibody; Zinc finger with KRAB and SCAN domains 3 antibody; ZKSC3_HUMAN antibody; ZKSCAN3 antibody; ZNF309 antibody; ZSCAN13 antibody; ZSCAN35 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zinc finger protein with KRAB and SCAN domains 3 protein (8-275AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZKSCAN3 Antibody (Product code: CSB-PA861122LA01HU) is Non-conjugated. For ZKSCAN3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA861122LB01HU	ZKSCAN3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861122LC01HU	ZKSCAN3 Antibody, FITC conjugated
Biotin	CSB-PA861122LD01HU	ZKSCAN3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.

Gene References into Functions

These results suggest that ZKSCAN3 plays an important role in bladder cancer outgrowth PMID: 27447553
ZKSCAN3 appears to promote colorectal tumor progression and invasion. ZKSCAN3 may facilitate hepatic metastasis of CRC associated with CEA particularly in cases with CEA-producing tumor PMID: 27127149
Suggest ZKSCAN3 as an autophagy master switch regulating the expression of a transcriptional network of genes integral to autophagy and lysosome biogenesis/function. PMID: 23434374
ZKSCAN3 induction represents a mechanism by which myeloma cells can induce cyclin D2 dysregulation and contribute to disease pathogenesis. PMID: 21057542
ZKSCAN3 adds to an expanding set of encoded products contributing to the progression of colorectal cancer PMID: 18519692
ZKSCAN3 regulates the expression of several genes favoring tumor progression including integrin beta 4 and VEGF PMID: 18940803

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Subcellular Location

Nucleus. Cytoplasm. Note=Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function.

Protein Families

Krueppel C2H2-type zinc-finger protein family

Database Links

HGNC: 13853

OMIM: 612791

KEGG: hsa:80317

STRING: 9606.ENSP00000252211

UniGene: Hs.380930

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Overview of Autophagy Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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