ZP2 Antibody

Datasheet

Code	CSB-PA29809A0Rb
Size	US$166
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Image	IHC image of CSB-PA29809A0Rb diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-PA29809A0Rb diluted at 1:200 and staining in paraffin-embedded human ovarian tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZP2 Polyclonal antibody

Uniprot No.

Q05996

Target Names

ZP2

Alternative Names

Zona pellucida sperm-binding protein 2 (Zona pellucida glycoprotein 2) (Zp-2) (Zona pellucida protein A) [Cleaved into: Processed zona pellucida sperm-binding protein 2], ZP2, ZPA

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zona pellucida sperm-binding protein 2 protein (440-716aa)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZP2 Antibody (Product code: CSB-PA29809A0Rb) is Non-conjugated. For ZP2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA29809B0Rb	ZP2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA29809C0Rb	ZP2 Antibody, FITC conjugated
Biotin	CSB-PA29809D0Rb	ZP2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.

Gene References into Functions

Mutations in ZP2 and ZP3 have dosage effects which can cause female infertility in humans. PMID: 28646452
ZP2(51-149) sperm-binding domain is necessary for human gamete recognition and penetration through the zona pellucida. PMID: 24934154
Studies indicate that eggs from mice lacking an ortholog of ZP2 do not contain a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts. PMID: 23417405
observed sequence variations in exons of ZP2 gene in women with infertility of unknown origin who exhibit abnormal zona pellucida; sperm-ovum interactions appear relatively normal in these patients [CASE REPORTS] PMID: 22889493
Of all zona pellucida (ZP) proteins, recombinant human ZPA demonstrates the highest binding activity toward acrosin PMID: 15950651
Exposure of sperm to ZP proteins promoted acrosomal exocytosis and changed motility patterns. PMID: 16407501
ZP proteins were detected in both the oocyte and the granulosa cells as early as the primordial follicle stage in the human. The detection of ZP proteins in the quiescent primordial follicle suggests that these proteins have been present since oogenesis. PMID: 18033806

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Subcellular Location

[Processed zona pellucida sperm-binding protein 2]: Zona pellucida.; Cell membrane; Single-pass type I membrane protein.

Protein Families

ZP domain family, ZPA subfamily

Tissue Specificity

Expressed in occytes(at protein level).

Database Links

HGNC: 13188

OMIM: 182888

KEGG: hsa:7783

STRING: 9606.ENSP00000219593

UniGene: Hs.73982

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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