def Antibody

^{Rare Species}

Datasheet

Code	CSB-PA017707HA01EOD
Size	US$166
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Image	Western Blot Positive WB detected in: DH5α whole cell lysate(20µg), DH5α whole cell lysate(10µg) All lanes: def antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 120s
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli def Polyclonal antibody

Uniprot No.

P0A6K3

Target Names

def

Alternative Names

def antibody; fms antibody; b3287 antibody; JW3248Peptide deformylase antibody; PDF antibody; EC 3.5.1.88 antibody; Polypeptide deformylase antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Peptide deformylase protein (2-169AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

The def Antibody (Product code: CSB-PA017707HA01EOD) is Non-conjugated. For def Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017707HB01EOD	def Antibody, HRP conjugated	ELISA
FITC	CSB-PA017707HC01EOD	def Antibody, FITC conjugated
Biotin	CSB-PA017707HD01EOD	def Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.

Gene References into Functions

Peptide deformylase has very fast association & dissociation kinetics which allows it to frequently sample ribosomes & ensure the processing of nascent chains after emergence. It competes with methionine aminopeptidase for ribosomal tunnel-exit binding sites. PMID: 23770820

Protein Families

Polypeptide deformylase family

Database Links

KEGG: ecj:JW3248

STRING: 316385.ECDH10B_3461

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