glpK Antibody, Biotin conjugated

Code CSB-PA18407D0Rb
Size US$166
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  • Western Blot
    Positive WB detected in: Rosseta bacteria lysate at 40μg, 20μg, 10μg, 5μg
    All lanes: glpK antibody at 2.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 57 kDa
    Observed band size: 57 kDa

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Product Details

Full Product Name
Rabbit anti-Escherichia coli glpK Polyclonal antibody
Uniprot No.
Target Names
glpK
Alternative Names
glpK antibody; b3926 antibody; JW3897Glycerol kinase antibody; EC 2.7.1.30 antibody; ATP:glycerol 3-phosphotransferase antibody; Glycerokinase antibody; GK antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli
Immunogen
Recombinant Escherichia coli Glycerol kinase protein (2-502AA)
Immunogen Species
Escherichia coli
Conjugate
Biotin
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.
Gene References into Functions
  1. point mutation promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism PMID: 24481126
  2. Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli. PMID: 21550976
  3. GKG230D displays increased enzymatic activity & decreased allosteric regulation by the glycolytic pathway intermediate fructose 1,6-bisphosphate (FBP) compared to wild-type GK. PMID: 17441732
  4. When the functional dimer for glycerol kinase is identified, it is determined that the domain-swap loop is not removed from the catalytic cleft upon dimer formation. PMID: 19819219
Protein Families
FGGY kinase family
Database Links
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