glpK Antibody

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Datasheet

Code	CSB-PA18407A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: Rosseta bacteria lysate at 80μg, 40μg, 20μg, 10μg All lanes: glpK antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli glpK Polyclonal antibody

Uniprot No.

P0A6F3

Target Names

glpK

Alternative Names

glpK antibody; b3926 antibody; JW3897Glycerol kinase antibody; EC 2.7.1.30 antibody; ATP:glycerol 3-phosphotransferase antibody; Glycerokinase antibody; GK antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Glycerol kinase protein (2-502AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

The glpK Antibody (Product code: CSB-PA18407A0Rb) is Non-conjugated. For glpK Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA18407B0Rb	glpK Antibody, HRP conjugated	ELISA
FITC	CSB-PA18407C0Rb	glpK Antibody, FITC conjugated
Biotin	CSB-PA18407D0Rb	glpK Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP.

Gene References into Functions

point mutation promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism PMID: 24481126
Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli. PMID: 21550976
GKG230D displays increased enzymatic activity & decreased allosteric regulation by the glycolytic pathway intermediate fructose 1,6-bisphosphate (FBP) compared to wild-type GK. PMID: 17441732
When the functional dimer for glycerol kinase is identified, it is determined that the domain-swap loop is not removed from the catalytic cleft upon dimer formation. PMID: 19819219

Protein Families

FGGY kinase family

Database Links

KEGG: ecj:JW3897

STRING: 316385.ECDH10B_4115

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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