lexA Antibody

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Datasheet

Code	CSB-PA363985HA01ENV
Size	US$166
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: lexA antibody at 3.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 39 kDa observed band size: 39 kDa
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli lexA Polyclonal antibody

Uniprot No.

P0A7C2

Target Names

lexA

Alternative Names

lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli LexA repressor protein (1-202AA)

Immunogen Species

Escherichia coli

Conjugate

Non-conjugated

The lexA Antibody (Product code: CSB-PA363985HA01ENV) is Non-conjugated. For lexA Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA363985HB01ENV	lexA Antibody, HRP conjugated	ELISA
FITC	CSB-PA363985HC01ENV	lexA Antibody, FITC conjugated
Biotin	CSB-PA363985HD01ENV	lexA Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The LexA antibody is prepared through the immunization of rabbits with the peptide corresponding to amino acids 1-202 of the recombinant E.coli LexA repressor protein and further purification from rabbit antiserum by protein G. It is a polyclonal antibody and exists as an unconjugated IgG. Its purity reaches up to 95%. It only reacts with the LexA protein of E.coli-origin. And this anti-LexA antibody has been validated for use in ELISA and WB analyses. In E.coli, the transcriptional repressor LexA controls the SOS response, which is a coordinated cellular response to DNA damage. The LexA protein inhibits gene expression by binding as a dimer to a 16-mer consensus sequence CTG-N10-CAG named SOS boxes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.

Gene References into Functions

The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response. PMID: 21576225
LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation. PMID: 21070632
NMR analysis of LexA catalytic domain in its active conformation PMID: 15929009
oligomerization of LexA is proposed to be a possible regulation mechanism of the Escherichia coli SOS regulon in response to environmental conditions resulting in acidic intracellular pH. Structural changes on LexA repressor promoted by acidic pH. PMID: 16701697
LexA binds to and represses the promoter of antC of coliphage N15. PMID: 17586637

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Protein Families

Peptidase S24 family

Database Links

KEGG: ecj:JW4003

STRING: 316385.ECDH10B_4232

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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