pagA Antibody

Datasheet

Code	CSB-PA320223XA01BQE
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: recombinant protein All lanes:pagA Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 89.6 kDa Observed band size: 90 kDa
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Bacillus anthracis pagA Polyclonal antibody

Uniprot No.

P13423

Target Names

pagA

Alternative Names

Protective antigen (PA) (Anthrax toxins translocating protein) (PA-83) (PA83) [Cleaved into: Protective antigen PA-20 (PA20), Protective antigen PA-63 (PA63)], pagA, pag

Raised in

Rabbit

Species Reactivity

Bacillus anthracis

Immunogen

Recombinant Bacillus anthracis pagA protein (30-764aa)

Immunogen Species

Bacillus anthracis

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.; Mediates the attachment to host cells by binding host cell receptors ANTXR1 and ANTXR2. Following host cell surface attachment, PA is cleaved by FURIN to generate the PA-63 (Protective antigen PA-63) form, which constitutes the mature form of the protein that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol.; Mature form that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol. Following attachment to host cell receptors and cleavage by FURIN, homooligomerizes to form ring-shaped oligomers that are in a pre-pore conformation, and associates with EF and LF. Toxin-leaded complexes are then endocytosed in a clathrin-dependent process, followed by a conformational change of oligomerized PA-63 from the pre-pore to pore state, which is triggered by the low pH in the endosome. Once active, the pore mediates unfolding of EF and LF, which pass through the pore and translocate into the host cytosol.

Gene References into Functions

PA is a calcium-dependent serine protease and that the protein potentially uses this proteolytic activity for receptor binding. PMID: 30052476
Stability of domain 4 of the anthrax toxin protective antigen and the effect of the VWA domain of CMG2 on stability PMID: 27874231
Data show that both mutant and native protective antigen vaccine preparations protected rabbits from lethal, aerosolized, spore challenge. PMID: 26207820
Data suggest that anthrax protective antigen (PA) expressing Semliki Forest virus (SFV) replicon DNA or viral replicon particles (VRP) vaccines may be suitable as candidate vaccines against anthrax. PMID: 25102364
N435C mutantion of anthrax protective antigen conferred dominant-negative inhibitory activity. PMID: 20213183
analysis of linear epitopes in Bacillus anthracis protective antigen bound by neutralizing antibodies PMID: 19617628

Hide All

Subcellular Location

[Protective antigen]: Secreted. Host cell membrane.; [Protective antigen PA-63]: Host cell membrane; Multi-pass membrane protein. Host endosome membrane; Multi-pass membrane protein.

Protein Families

Bacterial binary toxin family

Database Links

KEGG: bar:GBAA_pXO1_0164

Promotions

CUSABIO Premium Active Proteins 20% OFF SALE

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Recombinant Monkeypox Virus Proteins - Accelerate Monkeypox Research Proteolysis Targeting Chimera (PROTAC): A "Revolutionary" Technology in Small Molecule Drug Discovery! Protein Phosphorylation: The Dynamic Language of Cellular Regulation Tuberculosis--A Resurgent A "trembling" Parkinson's Disease Hematopoietic Stem Cell Surface Marker The Power and Promise of Stable Cell Lines The Recently Emerged New Swine Influenza Virus -- G4 EA-H1N1 TMPRSS2, an Activator for SARS-CoV-2 Invasion Cadherin-17 (CDH17): A Viable Target in Bispecific Antibodies or CAR-T Therapy to Fight Many Gastrointestinal Cancers! What Is Tomato Mosaic Virus CADM1: an Immunoglobulin Superfamily Molecule, a New Tumor Suppressor Gene! How the Tumor Microenvironment Fuels Cancer's Deadly Evolution Nicotinic Acetylcholine Receptor α1 Subunit (CHRNA1): Structure, Functional Mechanisms, and Disease-Related Research Progress Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address