ptsH Antibody

Datasheet

Code	CSB-PA362538HA01BRJ
Size	US$166
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Image	Western Blot Positive WB detected in Recombinant protein All lanes: ptsH antibody at 2.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Bacillus subtilis ptsH Polyclonal antibody

Uniprot No.

P08877

Target Names

ptsH

Alternative Names

ptsH antibody; BSU13900 antibody; Phosphocarrier protein HPr antibody; Histidine-containing protein antibody

Raised in

Rabbit

Species Reactivity

Bacillus subtilis

Immunogen

Recombinant Bacillus subtilis Phosphocarrier protein HPr protein (2-88AA)

Immunogen Species

Bacillus subtilis

Conjugate

Non-conjugated

The ptsH Antibody (Product code: CSB-PA362538HA01BRJ) is Non-conjugated. For ptsH Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA362538HB01BRJ	ptsH Antibody, HRP conjugated	ELISA
FITC	CSB-PA362538HC01BRJ	ptsH Antibody, FITC conjugated
Biotin	CSB-PA362538HD01BRJ	ptsH Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:2000-1:10000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA domain.; P-Ser-HPr interacts with the catabolite control protein A (CcpA), forming a complex that binds to DNA at the catabolite response elements cre, operator sites preceding a large number of catabolite-regulated genes. Thus, P-Ser-HPr is a corepressor in carbon catabolite repression (CCR), a mechanism that allows bacteria to coordinate and optimize the utilization of available carbon sources. P-Ser-HPr also plays a role in inducer exclusion, in which it probably interacts with several non-PTS permeases and inhibits their transport activity.

Gene References into Functions

Results show that in addition to the phosphorylase activity of the HPr kinase/phosphorylase, the serine/threonine protein phosphatase PrpC uses HPr(Ser-P) as a target. PMID: 17693724

Subcellular Location

Cytoplasm.

Protein Families

HPr family

Database Links

KEGG: bsu:BSU13900

STRING: 224308.Bsubs1_010100007716

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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