Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
rgpA; rgp1Gingipain R1; EC 3.4.22.37; Arg-gingipain; Gingipain 1; RGP-1
Species
Porphyromonas gingivalis
Expression Region
228-720aa
Target Protein Sequence
YTPVEEKQNGRMIVIVAKKYEGDIKDFVDWKNQRGLRTEVKVAEDIASPVTANAIQQFVKQEYEKEGNDLTYVLLVGDHKDIPAKITPGIKSDQVYGQIVGNDHYNEVFIGRFSCESKEDLKTQIDRTIHYERNITTEDKWLGQALCIASAEGGPSADNGESDIQHENVIANLLTQYGYTKIIKCYDPGVTPKNIIDAFNGGISLVNYTGHGSETAWGTSHFGTTHVKQLTNSNQLPFIFDVACVNGDFLFSMPCFAEALMRAQKDGKPTGTVAIIASTINQSWASPMRGQDEMNEILCEKHPNNIKRTFGGVTMNGMFAMVEKYKKDGEKMLDTWTVFGDPSLLVRTLVPTKMQVTAPAQINLTDASVNVSCDYNGAIATISANGKMFGSAVVENGTATINLTGLTNESTLTLTVVGYNKETVIKTINTNGEPNPYQPVSNLTATTQGQKVTLKWDAPSTKTNATTNTARSVDGIRELVLLSVSDAPELLRS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Recombinant Porphyromonas gingivalis Gingipain R1 (rgpA) with N-terminal 6xHis-tags is a partial protein expressed in yeast. Its encoding region corresponds to 228-720aa of rgpA of Porphyromonas gingivalis-origin. This recombinant rgpA protein was validated through SDS-PAGE and LC-MS/MS Analyses. It has a purity of over 90% and a molecular weight of 56.0 kDa. This rgpA is in-stock so that there is no waiting period for protein production. Based on rgpA's roles, this rgpA protein can find studies of periodontitis pathogenesis.
Arg-x-specific proteinase and adhesin (rgpA) is one of Porphyromonas gingivalis-expressed gingipains that is a unique type of cysteine proteases. RgpA is a critical virulence factor of P. gingivalis, an oral anaerobic bacterium causing periodontitis and gingivitis. Emerging evidence has shown that LPSs and RgpA of P. gingivalis can affect both clot structure and mechanics, which is of importance for clotting and clot formation when these inflammations enter into circulation.
