Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

NPPB

Uniprot No.

P16860

Research Area

Signal Transduction

Alternative Names

ANFB_HUMAN; BNP; BNP(1-32); BNP(5-29); BNP-32; Brain type natriuretic peptide; Gamma brain natriuretic peptide; Gamma-brain natriuretic peptide; Natriuretic peptide precursor B; nppb; NT proBNP

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

37-71aa

Target Protein Sequence

DLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

31.0kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Discover the benefits of our premium Recombinant Human NPPB protein, an essential tool for researchers studying signal transduction. Natriuretic peptides B is expressed in E. coli and features a partial protein sequence (37-71aa) to provide robust results across a range of research applications.

Equipped with an N-terminal GST-tag, this Recombinant Human NPPB protein offers straightforward purification and detection for streamlined experimental workflows. With a purity greater than 90% as determined by SDS-PAGE, you can trust in the quality of this protein for all your signal transduction research needs. Available in both liquid and lyophilized powder forms, our Recombinant Human NPPB protein provides flexibility and adaptability for your specific research requirements.

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Target Background

Function

Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.; May have a role in cardio-renal homeostasis. Able to promote the production of cGMP.

Gene References into Functions

NT-BNP is elevated in fetal aortic stenosis. PMID: 29327361
Combinative index (arterial lactate, NT-proBNP and CRP) could serve as a valuable predictor for the 28-day mortality rate in patients with sepsis. PMID: 30158031
Patients diagnosed with portopulmonary hypertension (POPH) had significantly higher BNP levels in comparison with those patients without. In contrast, PELD/MELD scores showed an almost negligible correlation with the BNP level. Liver transplantation was successful in 3 asymptomatic biliary atresia patients with POPH who had high BNP levels despite the low PELD/MELD scores. PMID: 29733490
NT-proBNP was associated with poor outcomes for patients with acute respiratory distress syndrome, and its level predicted mortality. PMID: 28322314
Levels of N-terminal pro-brain-type natriuretic peptide increases in response to exercise in patients with pulmonary arterial hypertension. PMID: 29334941
Admission NT-proBNP is an independent predictor for short- and long-term adverse events in Takotsubo syndrome patients. PMID: 29675571
Report a weak correlation between B-type natriuretic peptide and within-visit blood pressure variability. PMID: 29604077
Lung ultrasound, serum B-type natriuretic peptide levels, and echocardiography can be used for outcome prediction in patients with acute heart failure. PMID: 29532155
Data suggest that intact FGF23 level in plasma is independent predictor of cardiovascular death in patients with heart failure and provides added value to standard of care, natriuretic peptide (NT-proBNP) plasma level, for risk estimation. This study was conducted in Belgium. (FGF23 = fibroblast growth factor 23; NT-proBNP = aminoterminal pro-B-type natriuretic peptide) PMID: 30205090
Report decreased NT-proBNP levels in heart failure patients in contemporary heart failure management programs. PMID: 29656687
NT-proBNP may have a role in myocardial conduction abnormalities in patients with diabetes mellitus PMID: 29121498
recombinant human brain natriuretic peptide can regulate expression of IL-6, TNF-alpha, and IL-10 in lipopolysaccharide-activated RAW 264.7 cells. PMID: 29754152
n elderly patients with symptoms of heart failure ST2 was not superior to NTproBNP to predict all cause or cardiovascular mortality. Furthermore, it is unclear if the combination of ST2 and NTproBNP will improve long-term prognostication beyond what is achieved by NTproBNP alone. PMID: 29355441
current cutoffs for diagnosing HF for NT-proBNP and BNP have relatively low diagnostic concordance and correlation, particularly among patients with chronic kidney disease PMID: 30111510
Elevated plasma NT-proBNP and KIM-1 in patients with stage 5 chronic kidney disease (CKD5) undergoing maintenance hemodialysis (HD) and HF (+) (heart failure)CKD5-HD patients suggest that natriuretic peptides and KIM-1 may contribute to the pathogenesis of HF in CKD5-HD patients. PMID: 28990414
Increased levels of NT-proBNP seems to be a better biomarker of multidrug antihypertensive therapy requirement than CT-proAVP. PMID: 29190613
Baseline NT-proBNP concentration was associated with short-term stroke among patients hospitalized with acute heart filure. Stroke risk assessment models should consider incorporation of NT-proBNP measurement. PMID: 28905172
Suggest potential role for BNP as a protective endogenous factor against cerebral ischemia. PMID: 29203579
In children with chronic kidney disease, BNP is an indicator of heart failure correlating with renal function parameters and left ventricular mass index. PMID: 29775443
Data indicate that B-type natriuretic peptide (BNP) was outcome associated in acute heart failure patients. PMID: 28132515
Combination of coronary artery calcium score and NT-proBNP could be used to identify a group of coronary artery disease patients where MPI could be averted with a high degree of diagnostic safety. PMID: 27005324
NT-pro-BNP was not significantly different between subjects with 1-2 stenotic coronary arteries compared to those with 3 stenotic coronary arteries. PMID: 28845719
Serum levels of ST2, IL-33 and BNP were independent risk factors for major adverse cardiovascular events in acute myocardial infarction after percutaneous coronary intervention. PMID: 28623858
Circulating NT-proBNP levels are lower in overweight patients, even in those with diabetes, compared to controls. PMID: 28946871
Report usefulness of point-of-care test for NT-proBNP in primary care for the diagnosis of heart failure. PMID: 29263170
In-hospital mortality in patients with sepsis and low risk of sepsis-related organ failure was associated with NT-proBNP > 1,330 pg/ml. PMID: 28473224
The results of the present study suggest the possible use of the molecular pathology of BNP for the determination of the terminal cardiac function in SCD and analysis of its death mechanism in forensic practice. PMID: 28765973
In patients with a recent acute coronary syndrome and type 2 diabetes mellitus, BNP and NT-proBNP were powerful predictors of cardiovascular outcomes beyond heart failure and death, ie, were also predictive of MI and stroke. PMID: 28554908
Data suggest that subjects with morbid obesity exhibit down-regulation of serum levels of BNP compared to control subjects. This study was conducted in Italy. PMID: 27426417
Increased baseline values of BNP (and BigET-1) after acute myocardial infarction, were independently predictive of future cardiovascular events. PMID: 28284500
Patients with acute exacerbation of COPD and elevated NT-pro-BNP levels had increased hospital length of stay and need for intensive care. PMID: 28458528
BNP blood level is a biomarker for the the severity of hand-foot-mouth disease PMID: 28962547
Plasma levels of NT-pro-BNP were significantly higher in hypertrophic cardiomyopathy (HCM) patients with more advanced disease. Elevated NT-pro-BNP not only reflects the diastolic impairment of the LV, but it might also be the result of cardiac ischemia in patients with HCM. PMID: 28336204
Increased preoperative brain natriuretic peptide concentration was associated with risk of serious adverse events in pediatric cardiac surgery patients. PMID: 27919715
NPPB SNPs were not associated with insulin resistance in older adults. PMID: 27101535
Higher blood levels of NT-proBNP was associated with incident cognitive impairments. PMID: 27567834
In people with Type 2 diabetes, high baseline B-type natriuretic peptide levels were significantly associated with future atrial fibrillation development. PMID: 26173591
Plasma BNP levels were independently associated with functional outcomes 3 months after stroke onset in atrial fibrillation patients. PMID: 28253498
Short-term follow-up BNP and percent change in BNP level are significant prognostic factors of all-cause mortality in hospitalized heart failure patients. PMID: 28774268
NT-proBNP showed an incremental prognostic value for prediction of adverse outcomes when added to the clinical and echocardiographic parameters in patients with left ventricular dysfunction. PMID: 28349264
BNP gene body hypermethylation is associated with the risk of rheumatic heart disease (RHD), and also influenced by the warfarin anticoagulant therapy of RHD patients after surgery, which could represent novel and promising targets for therapeutic development. PMID: 27920275
IL-6 receptor blockade has an effect on high-sensitivity troponin T and NT-proBNP in rheumatoid arthritis PMID: 27744141
An exercise-associated increase in hs-cTnT and NT-proBNP occurred in response to a 60-minute maximal swimming test that was independent of pubertal status/adolescent vs adults. The present data also suggests that baseline and postexercise hs-cTnT values are higher in male compared with female, with no sex differences in NT-proBNP values. PMID: 29173327
An increase in NT-proBNP is a strong predictor of outcome in patients with left ventricular non-compaction cardiomyopathy. PMID: 28268080
Brain Natriuretic Peptide levels increased as heart failure duration increased. PMID: 28268084
In this hospitalized heart failure cohort, atrial fibrillation/flutter status did not influence post-discharge NT-proBNP trajectory. PMID: 27748006
NT-proBNP and BNP levels were statistically higher in patients with heart failure and anemia compared to patients with heart failure and without anemia. PMID: 28587950
The rs198389 G allele in the NPPB promoter is associated with elevated levels of NT-proBNP throughout adult life, reduced blood pressure, hypertension and cardiovascular mortality, and increased lifespan. PMID: 28341776
Atrial fibrillation risk discrimination was not significantly improved by the addition of NT-proBNP levels to the Framingham AF risk model. PMID: 27194537
Combination of high serum BNP levels, along with high soluble AXL had greater predictive value for heart failure than BNP alone. PMID: 27718443

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Subcellular Location

[NT-proBNP]: Secreted.; [proBNP(3-108)]: Secreted.; [Brain natriuretic peptide 32]: Secreted.; [BNP(3-32)]: Secreted.

Protein Families

Natriuretic peptide family

Tissue Specificity

[Brain natriuretic peptide 32]: Detected in the cardiac atria (at protein level). Detected in the kidney distal tubular cells (at protein level).

Database Links

HGNC: 7940

OMIM: 600295

KEGG: hsa:4879

STRING: 9606.ENSP00000365651

UniGene: Hs.219140

Code	CSB-EP016021HU(A5)
Abbreviation	Recombinant Human NPPB protein, partial
MSDS	MSDS Datasheet
Size	$224
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Recombinant Human natriuretic peptide B (NPPB), partial

Product Details

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Target Background

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