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Recombinant Clostridium perfringens Perfringolysin O (pfo)

Recombinant Clostridium perfringens Perfringolysin O (pfo)

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Code	CSB-EP314820CMB
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP314820CMB could indicate that this peptide derived from E.coli-expressed Clostridium perfringens (strain 13 / Type A) pfo.
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Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

pfo

Uniprot No.

P0C2E9

Research Area

Microbiology

Alternative Names

pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin

Species

Clostridium perfringens (strain 13 / Type A)

Source

E.coli

Expression Region

29-500aa

Target Protein Sequence

KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

68.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The E.coli-expressed recombinant Clostridium perfringens (strain 13/Type A) Perfringolysin O (pfo) verified by the LC-MS/MS Analysis is a full-length of mature protein with an N-terminal 6xHis-SUMO-tag. This in-stock recombinant protein harboring 29-500aa of Clostridium perfringens pfo has a high purity (>90%) measured by SDS-PAGE. Its calculated molecular mass is about 68.7 kDa. This pfo protein may be used in the research field of microbiology due to its origin.

Perfringolysin O (pfo), also called θ toxin, is a pore-forming toxin produced by an anaerobic, spore-forming Gram-positive bacterium Clostridium perfringens. Conformational alterations that influence oligomerization and initiate pore formation are triggered throughout pfo upon binding to cholesterol. Together with α toxin, pfo is implicated in the development of gas ganhrene and necrohemorrhagic enterities in calves.

Citations

Small cytosolic double-stranded DNA represses cyclic GMP-AMP synthase activation and induces autophagy Y Liu,Cell reports,2023
CD4+ T cell-mediated recognition of a conserved cholesterol-dependent cytolysin epitope generates broad antibacterial immunity L Ciacchi,Immunity,2023
Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes A Gainullina,Cell reports,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 3 reviews

Submit a Review here

Applications : Flow cytometry analysis

Review: Flow cytometry analysis of DC staining with cholesterol-dependent cytolysin perfringolysin O (PFO) that indicates the level of cholesterol in the cell membrane.

By Anonymous

Applications : /

Review: HLA-DP*04/ECT tetramer-binding CD4+ T cells were clearly identifiable in 6/10, 4/10, 4/10, and 7/10 cases following stimulation with Ply, LLO, PFO, or SLO, respectively.

By Anonymous

Applications : In vitro cGAS activity assay

Review: cGAMP binds to and activates STING to activate IκB kinase and TANK-binding kinase 1, thereby inducing the expression of interferons and other cytokines.

By Anonymous

Target Background

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein.

Protein Families

Thiol-activated cytolysin family

Database Links

KEGG: cpe:CPE0163

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