Greater than 90% as determined by SDS-PAGE.
pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
Clostridium perfringens (strain 13 / Type A)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The E.coli-expressed recombinant Clostridium perfringens (strain 13/Type A) Perfringolysin O (pfo) verified by the LC-MS/MS Analysis is a full-length of mature protein with an N-terminal 6xHis-SUMO-tag. This in-stock recombinant protein harboring 29-500aa of Clostridium perfringens pfo has a high purity (>90%) measured by SDS-PAGE. Its calculated molecular mass is about 68.7 kDa. This pfo protein may be used in the research field of microbiology due to its origin.
Perfringolysin O (pfo), also called θ toxin, is a pore-forming toxin produced by an anaerobic, spore-forming Gram-positive bacterium Clostridium perfringens. Conformational alterations that influence oligomerization and initiate pore formation are triggered throughout pfo upon binding to cholesterol. Together with α toxin, pfo is implicated in the development of gas ganhrene and necrohemorrhagic enterities in calves.