Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
| Code | CSB-EP314820CMB |
| Size | $2466 |
| Image |
|
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The E.coli-expressed recombinant Clostridium perfringens (strain 13/Type A) Perfringolysin O (pfo) verified by the LC-MS/MS Analysis is a full-length of mature protein with an N-terminal 6xHis-SUMO-tag. This in-stock recombinant protein harboring 29-500aa of Clostridium perfringens pfo has a high purity (>90%) measured by SDS-PAGE. Its calculated molecular mass is about 68.7 kDa. This pfo protein may be used in the research field of microbiology due to its origin. Perfringolysin O (pfo), also called θ toxin, is a pore-forming toxin produced by an anaerobic, spore-forming Gram-positive bacterium Clostridium perfringens. Conformational alterations that influence oligomerization and initiate pore formation are triggered throughout pfo upon binding to cholesterol. Together with α toxin, pfo is implicated in the development of gas ganhrene and necrohemorrhagic enterities in calves. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | pfo |
| Uniprot No. | P0C2E9 |
| Research Area | Microbiology |
| Alternative Names |
pfo; pfoA; pfoR; CPE0163; Perfringolysin O; Theta-toxin; Thiol-activated cytolysin
|
| Species | Clostridium perfringens (strain 13 / Type A) |
| Source | E.coli |
| Expression Region | 29-500aa |
| Target Protein Sequence | KDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 68.7kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info |
N-terminal 6xHis-SUMO-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
Applications : Flow cytometry analysis
Review: Flow cytometry analysis of DC staining with cholesterol-dependent cytolysin perfringolysin O (PFO) that indicates the level of cholesterol in the cell membrane.
By Anonymous
| Function |
A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
|
| Subcellular Location | Secreted. Host cell membrane; Multi-pass membrane protein. |
| Protein Families | Thiol-activated cytolysin family |
| Database Links |
KEGG: cpe:CPE0163 |
