pfo Antibody

Code CSB-PA314820HA01CMB
Size US$166
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Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: pfo antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56 kDa
    Observed band size: 56 kDa
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Product Details

Full Product Name
Rabbit anti-Clostridium perfringens pfo Polyclonal antibody
Uniprot No.
Target Names
pfo
Alternative Names
pfo antibody; pfoA antibody; pfoR antibody; CPE0163 antibody; Perfringolysin O antibody; Theta-toxin antibody; Thiol-activated cytolysin antibody
Raised in
Rabbit
Species Reactivity
Clostridium perfringens
Immunogen
Recombinant Clostridium perfringens Perfringolysin O protein (29-500AA)
Immunogen Species
Clostridium perfringens
Conjugate
Non-conjugated

The pfo Antibody (Product code: CSB-PA314820HA01CMB) is Non-conjugated. For pfo Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA314820HB01CMB pfo Antibody, HRP conjugated ELISA
FITC CSB-PA314820HC01CMB pfo Antibody, FITC conjugated
Biotin CSB-PA314820HD01CMB pfo Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Subcellular Location
Secreted. Host cell membrane; Multi-pass membrane protein.
Protein Families
Thiol-activated cytolysin family
Database Links

KEGG: cpe:CPE0163

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