Name: Recombinant Mycobacterium tuberculosis Putative amidase AmiB2 (amiB2)
Brand: CUSABIO
SKU: CSB-EP351872MVZ

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

amiB2

Uniprot No.

P9WQ97

Research Area

others

Alternative Names

amiB2; Rv1263; MTCY50.19cPutative amidase AmiB2; EC 3.5.1.4

Species

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Source

E.coli

Expression Region

1-462aa

Target Protein Sequence

MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLRAYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAIKDDVDIAGEVTTYGSAGHGPAATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFGATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSIRIPCTWCGLFGLKPQRDRISLEPHDGAWQGLSVNGPIARSVMDAALLLDATTTVPGPEGEFVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGALLRDLGHDVVVRDPDYPASTYANYLPRFFRGISDDADAQAHPDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDDVDVVVTPGAATGPSRIGAYQRRGAVSTLLLVVQRVPYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQIESARPWAHRRPSVS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

69.1kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I'm interested in the following protein, Bacterial amiB2 protein, catalog number, orb8243. I would like to confirm that it has biological activity (catalytically active). My intention is to use it to transform amide to acid

Thanks for your inquiry. The activity we haven't detected, so we can't gurantee. This protein is full length protein, and we purified it under the mild condition. It have activity in theory.

Can you tell me if this protein has been tested for bioactivity?

Very nice to receive your inquiry. The product details are confirmed as below.
Code: CSB-EP351872MVZ
Name: Recombinant Mycobacterium tuberculosis Putative amidase AmiB2(amiB2)
Expression Region: 1-462aa; Full Length
Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Expression Sequence:

Product Type: In-stock Recombinant Protein
Delivery Form: Lyophilized Powder
Buffer before Lyophilization: Lyophilized from a 0.2 μm filtered 10 mM Tris-HCl,1 mM EDTA,pH8.0
Endotoxin Removal: No
Aseptic Processing: No
Note: If your order size is .02mg*X vials or 0.02mg*X vials, we could deliver Lyophilized Powder directly. If not, we suggest to deliver Liquid Form.
We haven't tested the activity of this protein yet so we can't 100% guarantee that it is active. The protein we provide is full length of mature protein and purified under mild conditions,
which should have activity in theory.

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Code	CSB-EP351872MVZ
Size	US$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mycobacterium tuberculosis Putative amidase AmiB2 (amiB2)

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