Recombinant Agrobacterium sp. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)

In Stock
Code CSB-MP870830ALAE
MSDS
Size $440
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
aroA
Uniprot No.
Research Area
Others
Alternative Names
aroA3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS
Species
Agrobacterium sp. (strain CP4)
Source
Mammalian cell
Expression Region
1-455aa
Target Protein Sequence
MSHGASSRPATARKSSGLSGTVRIPGDKSISHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKSEDGDRLPVTLRGPKTPTPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGFGANLTVETDADGVRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILTLQEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPSMIDEYPILAVAAAFAEGATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRPDGKGLGNASGAAVATHLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
51.6kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Investigate the functionality of 3-phosphoshikimate 1-carboxyvinyltransferase with Recombinant Agrobacterium sp. (strain CP4) aroA protein. This full-length protein, spanning the 1-455aa expression region, is produced in mammalian cells to ensure proper folding and post-translational modifications. The N-terminal 6xHis-tag enables efficient purification, while the protein's purity is confirmed to be greater than 90% by SDS-PAGE analysis. Provided as a lyophilized powder, the recombinant Agrobacterium sp. aroA protein is a valuable tool for understanding the biochemical and structural properties of this critical enzyme involved in aromatic amino acid biosynthesis.

Customer Reviews and Q&A

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 Q&A
Q:

We previously ordered this protein CSB-MP870830ALAE multiple times and most recent order is from lot no. 03500.)
We would like same tag (N-terminal 6xHis-tagged), can you guarantee it?

A:
This is one of our best-selling proteins that has been frequently ordered by other customers, now we have 2mg of CSB-MP870830ALAE in stock.
We can guarantee to provide the tag of N-terminal 6xHis-tagged for this protein. In addition, if it's a re-order from the previous order, please remark this information when placing the order. We will try to keep all parameters as consistent as possible with previous order.

Target Background

Function
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
Subcellular Location
Cytoplasm.
Protein Families
EPSP synthase family
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