Recombinant Arabidopsis thaliana ABSCISIC ACID-INSENSITIVE 5-like protein 7 (ABF4)

Code CSB-YP885546DOA
MSDS
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Source Yeast
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Code CSB-EP885546DOA
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Source E.coli
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Code CSB-EP885546DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885546DOA
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Source Baculovirus
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Code CSB-MP885546DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ABF4
Uniprot No.
Alternative Names
ABF4; AREB2; BZIP38; At3g19290; MVI11.7ABSCISIC ACID-INSENSITIVE 5-like protein 7; ABA-responsive element-binding protein 2; Abscisic acid responsive elements-binding factor 4; ABRE-binding factor 4; bZIP transcription factor 38; AtbZIP38
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-431
Target Protein Sequence
MGTHINFNNL GGGGHPGGEG SSNQMKPTGS VMPLARQSSV YSLTFDELQN TLGGPGKDFG SMNMDELLKS IWTAEEAQAM AMTSAPAATA VAQPGAGIPP PGGNLQRQGS LTLPRTISQK TVDEVWKCLI TKDGNMEGSS GGGGESNVPP GRQQTLGEMT LEEFLFRAGV VREDNCVQQM GQVNGNNNNG FYGNSTAAGG LGFGFGQPNQ NSITFNGTND SMILNQPPGL GLKMGGTMQQ QQQQQQLLQQ QQQQMQQLNQ PHPQQRLPQT IFPKQANVAF SAPVNITNKG FAGAANNSIN NNNGLASYGG TGVTVAATSP GTSSAENNSL SPVPYVLNRG RRSNTGLEKV IERRQRRMIK NRESAARSRA RKQAYTLELE AEIEKLKKTN QELQKKQAEM VEMQKNELKE TSKRPWGSKR QCLRRTLTGP W
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as transcriptional activator in the ABA-inducible expression of LTI65/RD29B (AC Q04980). Binds specifically to the ABA-responsive element (ABRE) of the LTI65/RD29B (AC Q04980) gene promoter. Binds to the promoter of FREE1 and activates its transcription.
Gene References into Functions
  1. Besides enhancing salt stress and drought tolerance, constitutive expression of ABF4 increases tuber yield under normal and stress conditions, enhances storage capability and improves the processing quality of the tubers. PMID: 30143991
  2. the role of ABF1/3/4 in det1 germination phenotypes PMID: 29680877
  3. ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis PMID: 27373216
  4. AREB1, AREB2, and ABF3 are master transcription factors that regulate the ABRE-dependent expression of water-stress responsive genes in ABA signaling in response to water stresses in a cooperative manner. PMID: 19947981
  5. CPK32 is an ABA signaling component that regulates the ABA-responsive gene expression via ABF4. PMID: 16299177

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Subcellular Location
Nucleus.
Protein Families
BZIP family, ABI5 subfamily
Tissue Specificity
Expressed in roots, leaves, flowers and immatures siliques.
Database Links

KEGG: ath:AT3G19290

STRING: 3702.AT3G19290.3

UniGene: At.23993

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