Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DREB2C

Uniprot No.

Q8LFR2

Research Area

Others

Alternative Names

DREB2C; ERF048; At2g40340; T07M07.24; T3G21.11Dehydration-responsive element-binding protein 2C; Protein DREB2C

Species

Arabidopsis thaliana(Mouse-ear cress)

Source

E.coli

Expression Region

1-341aa

Target Protein Sequence

MPSEIVDRKRKSRGTRDVAEILRQWREYNEQIEAESCIDGGGPKSIRKPPPKGSRKGCMKGKGGPENGICDYRGVRQRRWGKWVAEIREPDGGARLWLGTFSSSYEAALAYDEAAKAIYGQSARLNLPEITNRSSSTAATATVSGSVTAFSDESEVCAREDTNASSGFGQVKLEDCSDEYVLLDSSQCIKEELKGKEEVREEHNLAVGFGIGQDSKRETLDAWLMGNGNEQEPLEFGVDETFDINELLGILNDNNVSGQETMQYQVDRHPNFSYQTQFPNSNLLGSLNPMEIAQPGVDYGCPYVQPSDMENYGIDLDHRRFNDLDIQDLDFGGDKDVHGST
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

43.9 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Constructing a plasmid that codes for the Arabidopsis thaliana(Mouse-ear cress) DREB2C protein (1-341aa) is the initial step to yield the recombinant Arabidopsis thaliana(Mouse-ear cress) DREB2C protein. The plasmid is then transferred into e.coli cells. Positive e.coli cells are selected and cultured for the protein expression. A N-terminal 10xHis tag is fused to the protein. The affinity purification is used to purify the protein, and SDS-PAGE analysis is carried out to verify the presence and assess the purity of the protein. The protein possesses a purity exceeding 85%.

Target Background

Function

Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and abscisic acid-inducible transcription.

Gene References into Functions

These results demonstrate that VOZ1 functions as a negative regulator of heat stress (HS)-inducible DREB2C signaling by blocking access to the AP2 DNA-binding domain of DREB2C. PMID: 29536220
DREB2C functions as transcriptional activator that promotes NaCl tolerance, in part through upregulation of the stress-responsive gene COR15A. PMID: 24737413
results suggest that DREB2C is a stress- and ABA-inducible gene that acts as a positive regulator of ABA biosynthesis in germinating seeds through activating NCED9 expression PMID: 25150152
We propose that AtCYS4 functions in thermotolerance under the control of the DREB2C cascade PMID: 23868510
The TATA-proximal region and some latent trans-acting factors may cooperate in heat shock-induced activation of the Arabidopsis DREB2C promoter. PMID: 22716647
These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis. PMID: 22286229
these results indicate that DREB2C and HsfA3 are key players in regulating the heat tolerance of Arabidopsis. PMID: 20849812
Overexpression of DREB2C affects the expression of stress-responsive genes and affects ABA sensitivity. PMID: 20395451
identify possible targets of DREB2C transcription factors at the protein level PMID: 19830397
Data suggest that DREB2C is a regulator of heat stress tolerance in Arabidopsis. PMID: 17716623

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Subcellular Location

Nucleus.

Protein Families

AP2/ERF transcription factor family, ERF subfamily

Database Links

KEGG: ath:AT2G40340

STRING: 3702.AT2G40340.1

UniGene: At.37068

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Code	CSB-EP850687DOA
Abbreviation	Recombinant Mouse-ear cress DREB2C protein
MSDS	MSDS Datasheet
Size	US$388
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Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 2C (DREB2C)

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