Recombinant Arabidopsis thaliana Gibberellin 20 oxidase 2 (20ox2)

Code CSB-YP657188DOA
MSDS
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Source Yeast
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Code CSB-EP657188DOA
MSDS
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Source E.coli
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Code CSB-EP657188DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657188DOA
MSDS
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Source Baculovirus
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Code CSB-MP657188DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GA20OX2
Uniprot No.
Alternative Names
GA20OX2; 20ox; At2353; At5g51810; MIO24.5Gibberellin 20 oxidase 2; EC 1.14.11.-; GA 20-oxidase 2; Gibberellin C-20 oxidase 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-378
Target Protein Sequence
MAILCTTTSP AEKEHEPKQD LEKDQTSPLI FNPSLLNLQS QIPNQFIWPD EEKPSIDIPE LNVPFIDLSS QDSTLEAPRV IAEACTKHGF FLVVNHGVSE SLIADAHRLM ESFFDMPLAG KQKAQRKPGE SCGYASSFTG RFSTKLPWKE TLSFQFSNDN SGSRTVQDYF SDTLGQEFEQ FGKVYQDYCE AMSSLSLKIM ELLGLSLGVN RDYFRGFFEE NDSIMRLNHY PPCQTPDLTL GTGPHCDPSS LTILHQDHVN GLQVFVDNQW QSIRPNPKAF VVNIGDTFMA LSNGIFKSCL HRAVVNRESA RKSMAFFLCP KKDKVVKPPS DILEKMKTRK YPDFTWSMFL EFTQKHYRAD VNTLDSFSNW VITNNNPI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product. Involved in the promotion of the floral transition, fertility and silique elongation, but plays only a minor role in elongation of seedling organs. Acts redundantly with GA20OX1.
Gene References into Functions
  1. DELLA-GAF1 complex is a main component of the Gibberellins feedback regulation of AtGA20ox2. PMID: 28916594
  2. The ga20ox2 mutation suppresses the elevated GA levels and partially suppresses the growth and early flowering phenotypes of svp mutants. PMID: 24979809
Protein Families
Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily
Tissue Specificity
Expressed in inflorescence and developing siliques. Detected in seeds, roots, cotyledons and leaves. In seeds, specifically detected at the rim of the embryo and the outer integument.
Database Links

KEGG: ath:AT5G51810

STRING: 3702.AT5G51810.1

UniGene: At.28053

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