Recombinant Arabidopsis thaliana Glucose-1-phosphate adenylyltransferase small subunit, chloroplastic (APS1)

Code CSB-YP345051DOA
MSDS
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Source Yeast
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Code CSB-EP345051DOA
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Source E.coli
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Code CSB-EP345051DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345051DOA
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Source Baculovirus
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Code CSB-MP345051DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
APS1
Uniprot No.
Alternative Names
APS1; ADG1; APS; At5g48300; K23F3.2Glucose-1-phosphate adenylyltransferase small subunit; chloroplastic; EC 2.7.7.27; ADP-glucose pyrophosphorylase; ADP-glucose synthase; AGPase B; Alpha-D-glucose-1-phosphate adenyl transferase
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
72-520
Target Protein Sequence
VSDSQNSQT CLDPDASSSV LGIILGGGAG TRLYPLTKKR AKPAVPLGAN YRLIDIPVSN CLNSNISKIY VLTQFNSASL NRHLSRAYAS NMGGYKNEGF VEVLAAQQSP ENPNWFQGTA DAVRQYLWLF EEHNVLEYLI LAGDHLYRMD YEKFIQAHRE TDADITVAAL PMDEQRATAF GLMKIDEEGR IIEFAEKPKG EHLKAMKVDT TILGLDDQRA KEMPFIASMG IYVVSRDVML DLLRNQFPGA NDFGSEVIPG ATSLGLRVQA YLYDGYWEDI GTIEAFYNAN LGITKKPVPD FSFYDRSAPI YTQPRYLPPS KMLDADVTDS VIGEGCVIKN CKIHHSVVGL RSCISEGAII EDSLLMGADY YETATEKSLL SAKGSVPIGI GKNSHIKRAI IDKNARIGDN VKIINSDNVQ EAARETDGYF IKSGIVTVIK DALIPTGTVI
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.
Gene References into Functions
  1. ACHT4 further reacted uniquely with the small subunit APS1, the first committed enzyme of the starch synthesis pathway, suggesting that it transfers the disulfides it receives from 2-Cys Prx to APS1 and turns off AGPase. PMID: 26424450
  2. Data show that APS1 (At5g48300) protein levels were five- to tenfold lower in adg1/APS1(C81S) lines than in control plants. PMID: 22098298
  3. Redox modification of ADP-glucose pyrophosphorylase in response to light is not a major determinant of fine regulation of transitory starch accumulation in Arabidopsis. PMID: 22210900
  4. adg1-1 and aps1 Arabidopsis mutants accumulate starch and ADP-glucose pyrophosphorylase in their leaves. PMID: 21624897
  5. Post-translational activation of ADP-glucose pyrophosphorylase [ADPG pyrophosphorylase(AGPase)] PMID: 15341628

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Subcellular Location
Plastid, chloroplast.
Protein Families
Bacterial/plant glucose-1-phosphate adenylyltransferase family
Tissue Specificity
Leaves.
Database Links

KEGG: ath:AT5G48300

STRING: 3702.AT5G48300.1

UniGene: At.670

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