Recombinant Arabidopsis thaliana Protein SOMBRERO (SMB)

Code CSB-YP878715DOA
MSDS
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Source Yeast
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Code CSB-EP878715DOA
MSDS
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Source E.coli
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Code CSB-EP878715DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878715DOA
MSDS
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Source Baculovirus
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Code CSB-MP878715DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SMB
Uniprot No.
Alternative Names
SMB; NAC033; At1g79580; F20B17.1; Protein SOMBRERO; NAC domain-containing protein 33; ANAC033
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-371
Target Protein Sequence
MEIGSSSTVA GGGQLSVPPG FRFHPTEEEL LYYYLKKKVS YEPIDLDVIR EVDLNKLEPW ELKEKCRIGS GPQNEWYFFS HKDKKYPTGT RTNRATAAGF WKATGRDKSI HLNSSKKIGL RKTLVFYTGR APHGQKTEWI MHEYRLDDSE NEIQEDGWVV CRVFKKKNHF RGFHQEQEQD HHHHHQYIST NNDHDHHHHI DSNSNNHSPL ILHPLDHHHH HHHIGRQIHM PLHEFANTLS HGSMHLPQLF SPDSAAAAAA AAASAQPFVS PINTTDIECS QNLLRLTSNN NYGGDWSFLD KLLTTGNMNQ QQQQQVQNHQ AKCFGDLSNN DNNDQADHLG NNNGGSSSSP VNQRFPFHYL GNDANLLKFP K
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription regulator. Together with BRN1 and BRN2, regulates cellular maturation of root cap. Represses stem cell-like divisions in the root cap daughter cells, and thus promotes daughter cell fate. Inhibits expression of its positive regulator FEZ in a feedback loop for controlled switches in cell division plane. Promotes the expression of genes involved in secondary cell walls (SCW) biosynthesis.
Gene References into Functions
  1. FEZ and SOMBRERO (SMB), two plant-specific NAC-domain transcription factors, control the orientation of cell division plane in Arabidopsis root stem cells. [SOMBRERO] PMID: 19081078
  2. SMB, BRN1, and BRN2 act redundantly to drive cellular differentiation and promote maturation of the root cap and the cell wall separations needed to produce a functional root cap. PMID: 20197506
Subcellular Location
Nucleus.
Tissue Specificity
Accumulates in maturing root cap cells, in both COL and LRC cells.
Database Links

KEGG: ath:AT1G79580

STRING: 3702.AT1G79580.1

UniGene: At.50052

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