Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1)

Code CSB-YP660355DOA
MSDS
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Source Yeast
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Code CSB-EP660355DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP660355DOA
MSDS
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Source Baculovirus
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Code CSB-MP660355DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RGF1
Uniprot No.
Alternative Names
RGF1; At5g60810; MAE1.14; Root meristem growth factor 1; AtRGF1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
104-116
Target Protein Sequence
DYSNPGH HPPRHN
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a peptide hormone recognized by receptors (e.g. RGI1 and RGI2) to trigger signaling events including the regulation of RITF1 expression and leading to the production of reactive oxygen species (ROS) in roots to modulate meristem size. Signaling peptide (root growth factor) that maintains the postembryonic root stem cell niche in a PIN2-traffic dependent manner by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT), mainly at the post-transcriptional level. Maintains the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (e.g. PLT1 and PLT2), mainly at the post-transcriptional level. Influences circumferential cell number in the root meristem in response to Pi-deprivation. Regulates the pattern of root growth and lateral root development by modulating the length and the number of cortical cells in the root apical meristem (RAM), and the anticlinal asymmetric cell divisions in lateral root initiation cells.
Gene References into Functions
  1. A clade of leucine-rich repeat receptor-like kinases, designated as RGF1 INSENSITIVE 1 (RGI1) to RGI5, serving as receptors of RGF1, were identified. PMID: 27229312
  2. RGF2 influenced the longitudinal growth rate in the primary root in response to inorganic phosphate deprivation, whereas RGF1 affected circumferential cell number in the root meristem. PMID: 25856240
  3. root meristem growth factor 1(At5g60810 peptide) required for maintenance of root stem cell niche and transit amplifying cell proliferation; it defines expression levels and patterns of stem cell transcription factor PLETHORA PMID: 20798316
Subcellular Location
[GLV11p]: Secreted.
Protein Families
RGF family
Tissue Specificity
Expressed in root tips, only in the quiescent center and the columella stem cells.
Database Links

KEGG: ath:AT5G60810

STRING: 3702.AT5G60810.1

UniGene: At.55649

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