Recombinant Arabidopsis thaliana Sorting nexin 1 (SNX1)

Code CSB-YP022353DOA
MSDS
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Source Yeast
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Code CSB-EP022353DOA
MSDS
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Source E.coli
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Code CSB-EP022353DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022353DOA
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Source Baculovirus
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Code CSB-MP022353DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
SNX1; VPS5; At5g06140; K16F4.11; MBL20; Sorting nexin 1; AtSNX1; Vacuolar protein sorting-associated protein 5 homolog
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-402
Target Protein Sequence
MESTEQPRNI SGSMQSPRSP SSHPYLSVSV TDPVKLGNGV QAYISYRVIT KTNLPEYQGP EKIVIRRYSD FVWLRDRLFE KYKGIFIPPL PEKSAVEKFR FSAEFIEMRR AALDIFVNRI ALHPELQQSE DLRTFLQADE ETMDRFRFQE TSIFKKPADL MQMFRDVQSK VSDAVLGKEK PVEETTADYE KLKHYIFELE NHLTEAQKHA YRLVKRHREL GQSLLDFGKA VKLLGACEGE PTGKAFSDLG TKSELLSIKL QKEAQQVLMN FEEPLKDYVR YVQSIKATIA ERGTAFKQHC ELSETTKLKE INLDKLMLTR SDKVGEAEIE YREIKAESEE ATRRFERIVK RMEDEIVRFQ EQKTEEMGVA FHQFAKGQAR LANSVADAWR SLLPKLEASY SV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in vesicular protein sorting. Acts at the crossroads between the secretory and endocytic pathways. Is involved in the endosome to vacuole protein transport via its interaction with the BLOS1/2 proteins and, as component of the membrane-associated retromer complex, is also involved in endosome-to-Golgi retrograde transport. Required for the auxin-carrier protein PIN2 sorting to the lytic vacuolar pathway and the trafficking of several plasma membrane proteins. Also involved in the efficient sorting of seed storage protein globulin 12S.
Gene References into Functions
  1. The Arabidopsis SNX1 protein is involved in recycling the iron transporter IRT1, thus promoting its presence at the plasma membrane. PMID: 29219710
  2. High temperature selectively promotes the retrieval of PIN2 from late endosomes and sorts them to the plasma membrane through an endosomal trafficking pathway dependent on SORTING NEXIN1. PMID: 24003052
  3. SNX1 phosphorylation is required for Arabidopsis seedling growth. PMID: 23328941
  4. A combination of immunoelectron and fluorescence microscopy show that sorting nexin 1 (SNX1)localize to the trans-Golgi network (TGN), which is considered to represent the early endosome of plants. PMID: 19796370
  5. Arabidopsis thaliana SORTING NEXIN 1 (AtSNX1) is involved in the auxin pathway and that PIN2, but not PIN1 or AUX1, is transported through AtSNX1-containing endosomes [AtSNX1] PMID: 16936718

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Subcellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Sorting nexin family
Tissue Specificity
Ubiquitously expressed.
Database Links

KEGG: ath:AT5G06140

STRING: 3702.AT5G06140.1

UniGene: At.27700

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