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Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 11 (UBC11)

Recombinant Arabidopsis thaliana Ubiquitin-conjugating enzyme E2 11 (UBC11)

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Code	CSB-EP339530DOA
Abbreviation	Recombinant Mouse-ear cress UBC11 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

UBC11

Uniprot No.

P35134

Research Area

Cell Biology

Alternative Names

E2 ubiquitin-conjugating enzyme 11 Ubiquitin carrier protein 11 Ubiquitin-conjugating enzyme E2-17 kDa 11 Ubiquitin-protein ligase 11

Species

Arabidopsis thaliana (Mouse-ear cress)

Source

E.coli

Expression Region

1-148aa

Target Protein Sequence

MASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYPFKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRSKYESTARSWTQKYAMG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

20.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Arabidopsis thaliana (Mouse-ear cress) UBC11 protein is made by following sequential steps: inserting a DNA fragment encoding the Arabidopsis thaliana (Mouse-ear cress) UBC11 protein (1-148aa) into a plasmid vector, transferring this vector into e.coli cells, selecting the positive cells, culturing positive cells, inducing the protein expression, and collecting the UBC11 protein. The protein features a N-terminal 6xHis tag fusion. The recombinant UBC11 protein is subjected to affinity purification and SDS-PAGE analysis. Its purity is over 90%.

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Target Background

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Protein Families

Ubiquitin-conjugating enzyme family

Tissue Specificity

Ubiquitously expressed. Mainly in petals.

Database Links

KEGG: ath:AT3G08690

STRING: 3702.AT3G08690.1

UniGene: At.202

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