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Recombinant Arachis hypogaea Allergen Ara h 1, clone P17, partial

Recombinant Arachis hypogaea Allergen Ara h 1, clone P17, partial

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Code	CSB-EP331779ANE
MSDS	MSDS
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Uniprot No.

P43237

Research Area

Others

Alternative Names

Allergen Ara h 1; clone P17; Allergen Ara h I; allergen Ara h 1

Species

Arachis hypogaea (Peanut)

Source

E.coli

Expression Region

26-216aa

Target Protein Sequence

KSPYRKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDTGATNQRHPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAEPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSEVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSKQFQNLQNHRIVQIEARPN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

39.0kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

I would like to know more about the aseptic and endotoxin removal. I would like to know the following:
1)If Aseptic processing is by heat sterilization?
2)Whether heat sterilization treatment will cause protein denaturation?

Thanks for your inquiry.
1. The aseptic process doesn't perform by heat sterilization, It is filtered and sterilized by a 0.22um sterile filter, which generally does not affect the protein structure activity.
2.We mainly use the PMB affinity chromatography (polymyxin B-agarose) method for endotoxin removal, which is mild and generally does not affect the protein structure activity;
then the LAL reagent is used to qualitatively detect endotoxin content. To ensure endotoxin levels are within 0.1 ng/ug (1 EU/ug).

Regarding the product RPC20274 CSB-EP331779ANE- Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial
Can this allergen be used in allergy animal model research?

This protein can be used in allergy animal model research. Here we provide the reference link: https://en.wikipedia.org/wiki/Ara_h1

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