Recombinant Atlantic salmon Vertebrate ancient opsin,Partial

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Code CSB-EP517517SWI
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Uniprot No.
Research Area
others
Alternative Names
Vertebrate ancient opsin
Species
Salmo salar (Atlantic salmon)
Source
E.coli
Expression Region
1-75aa
Target Protein Sequence
MDTLRIAVNGVSYNEASEIYKPHADPFTGPITNLAPWNFAVLATLMFVITSLSLFENFTVMLATYKFKQLRQPLN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
22.5kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-B2M-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

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Applications : Drug related studies

Review: Recombinant vertebrate ancient opsin was dissolved in 0.9% physiological saline, and each fish was injected with VAL-opsin [0.1 and 0.5 μg/g, body mass (BM)] at a volume of 10 μL/g BM.

By Anonymous

Applications : Drug treatment

Review: Changes in vertebrate ancient long opsin (VAL-opsin) mRNA expression levels upon light treatment (green LED(520 nm) (G) and a white fluorescent bulb (W)) in the hypothalamus after VAL-opsin (0.5 μg/g BM), cetrorelix (Cx) (0.5 μg/fish), or cetrorelix (Cx) (0.5 μg/fish) + VAL-opsin (0.5 μg/g BM) injections, and no injection as a control group (Cont.). Different letters indicate significant differences between groups with respect to simultaneous VAL-opsin injection (P < 0.05).

By Anonymous

Target Background

Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family, Opsin subfamily
Database Links

KEGG: sasa:100136521

UniGene: Ssa.192

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