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Recombinant Bacillus anthracis Thymidylate kinase (tmk)

Recombinant Bacillus anthracis Thymidylate kinase (tmk)

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Code	CSB-EP007226BQG
Abbreviation	Recombinant Bacillus anthracis Thymidylate kinase protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

tmk

Uniprot No.

C3LJ02

Research Area

Others

Alternative Names

tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Species

Bacillus anthracis (strain CDC 684 / NRRL 3495)

Source

E.coli

Expression Region

1-208aa

Target Protein Sequence

MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

27.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The full-length cDNA ORF of Bacillus anthracis Thymidylate kinase (tmk) was N-terminally tagged a 6xHis and then expressed in E.coli. The Recombinant Bacillus anthracis tmk obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, the tmk protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant tmk protein may be used to produce specific antibodies or find uses in the DNA synthesis-related studies.

Tmk is a key enzyme exerting an important role in DNA synthesis. It catalyzes the conversion of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), with ATP as the preferred phosphoryl donor and in the presence of Mg2+. It takes place at the junction of the de novo and salvage pathways for thymidine triphosphate (dTTP) synthesis. Tmk is thus regarded as a potential therapeutic target for the development of antibacterial, antiparasitic, and anticancer drugs.