Recombinant Bovine Coagulation factor IX (F9) (Active)

Factor IX plays a central role in the intrinsic coagulation pathway, and this recombinant bovine protein spanning residues 26–462 demonstrates robust catalytic function with a specific activity exceeding 30 pmol/min/µg against the fluorogenic substrate MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)NH2. The C-terminal His tag preserves active site accessibility while enabling straightforward purification, and the quantified activity data provides a validated foundation for kinetic parameter analysis including Km and kcat determination, substrate specificity profiling, and inhibitor screening with IC50 measurements. Dual-method purity assessment by SDS-PAGE and SEC-HPLC both exceeding 95%, combined with endotoxin levels below 1.0 EU/µg, supports accurate enzymatic assays where contaminants could confound rate measurements or interfere with drug candidate evaluation. This preparation is appropriate for use as a positive control in enzyme-linked assays and meets the quality thresholds commonly required for structural studies investigating coagulation cascade mechanisms.