Recombinant Bovine Fatty acid synthase (FASN), partial

Code CSB-YP008435BO
MSDS
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Source Yeast
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Code CSB-EP008435BO
MSDS
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Source E.coli
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Code CSB-EP008435BO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008435BO
MSDS
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Source Baculovirus
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Code CSB-MP008435BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FASN
Uniprot No.
Alternative Names
FASNFatty acid synthase; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase; EC 3.1.2.14)]
Species
Bos taurus (Bovine)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Gene References into Functions
  1. FASN single nucleotide polymorphisms associated with milk fatty acids in a Chinese Holstein cattle. PMID: 27468856
  2. g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle. PMID: 25597874
  3. Results indicate that Fatty acid synthase (FASN) may be used as a candidate gene affecting intramuscular fat content in Datong yaks. PMID: 25867409
  4. It was suggested that two cattle breeds have an advantage in terms of genotype and haplotype distribution of the FASN gene; a single polymorphism might be a potential marker for breed discrimination. PMID: 24745147
  5. study evaluated the contributions of polymorphisms of FASN and SCD genes on fatty acid composition in muscle in two different populations: 1189 and 1058 Japanese Black cattle from the Miyagi and the Yamagata populations PMID: 22497525
  6. The five exonic single nucleotide polymorphisms of g.12870, g.13126, g.15532, g.16907, and g.17924 in the FASN gene could change fatty acid contents. PMID: 21773945
  7. Results suggest that SCD and FASN are strong candidate genes influencing fatty acid composition in beef cattle. PMID: 22221030
  8. The effects of genetic polymorphisms of liver X receptor, alpha (LXR), stearoyl-CoA desaturase (SCD), Fatty acid synthase (FASN), and Fatty acid binding protein 4 (FABP4) were investigated on fatty acid composition in fat tissue of steers. PMID: 21615833
  9. In ruminants, FASN may be regulated between the ratio between two transcripts. The small transcript is mostly produced in tissues with low fatty acid synthesis. PMID: 16093723
  10. Polymorphisms within the FASN gene are associated with milk-fat content. PMID: 16734679
  11. SNPs in the FASN gene are associated with variation in the fatty acid composition of adipose fat and milk fat. PMID: 17242864
  12. The four exons in FASN that encode for the TE domain were sequenced, and three SNPs, AF285607:g.17924A>G, g.18663T>C and g.18727C>T, were identified. PMID: 18254736
  13. These results demonstrate that the single nucleotide polymorphism alters the bovine FASN promoter activity in vitro and the Sp1/Sp3 binding ability of the sequence. PMID: 18477667
  14. Results suggest that the mutations may contribute to the characteristic fatty acid composition of Japanese Black beef. PMID: 19291389

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Subcellular Location
Cytoplasm. Melanosome.
Database Links
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