Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), partial

Code CSB-YP005393BO
MSDS
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Source Yeast
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Code CSB-EP005393BO
MSDS
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Source E.coli
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Code CSB-EP005393BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005393BO
MSDS
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Source Baculovirus
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Code CSB-MP005393BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CHRNA7
Uniprot No.
Alternative Names
CHRNA7Neuronal acetylcholine receptor subunit alpha-7
Species
Bos taurus (Bovine)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Gene References into Functions
  1. We conclude that residues in the M2 segment and flanking regions contribute to the unusual properties of the CHRNA7 receptor PMID: 21790604
  2. Deletion of a small cytoplasmic fragment close to the fourth transmembrane segment of the nicotinic alpha7 receptor (Glu437 to Arg447) abolished membrane expression PMID: 21729697
  3. Data show that basic residues at positions 208 and 210 are necessary for the assembly of alpha7 receptors. PMID: 21420959
  4. Cultured bovine retinal pigment epitheliumcells expressed nicotinic receptors containing alpha7 subunit PMID: 20932358
  5. Loop 9 has a different role in the function of homomeric and heteromeric receptors. PMID: 20043866
  6. Loop 3 and its docking to the alpha-helix is an important requirement for receptor assembly of the alpha7 nicotinic receptor. PMID: 19840217
  7. Bovine chromaffin cells have an alpha3alpha7beta4 nicotinic receptor PMID: 12438113
  8. a network of interactions formed by residues Lys-46, Asp-128, Asp-135, Asp-266, and possibly others appears to link agonist binding to channel gating PMID: 15611071
  9. involvement of Lys145 in the early steps of channel activation by acetylcholine PMID: 16129734
  10. slowly desensitizing mutant alpha7L248T could be an interesting tool for the study of channel activation in alpha7 receptors PMID: 19063861

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Subcellular Location
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Protein Families
Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-7/CHRNA7 sub-subfamily
Tissue Specificity
At least in chromaffin cells.
Database Links

KEGG: bta:282178

STRING: 9913.ENSBTAP00000020942

UniGene: Bt.88

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