Recombinant Bovine Osteopontin (SPP1)

Code CSB-YP022603BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP022603BO
MSDS
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Source E.coli
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Code CSB-EP022603BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022603BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022603BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPP1
Uniprot No.
Alternative Names
SPP1; OPN; Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1
Species
Bos taurus (Bovine)
Expression Region
17-278
Target Protein Sequence
LPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL DLDHKSEEDK HLKIRISHEL DSASSEVN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite (Probable). Appears to form an integral part of the mineralized matrix (Probable). Probably important to cell-matrix interaction (Probable).; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Gene References into Functions
  1. study first addressed in detail the LF/fOPN interaction and proposed a LF:fOPN 4/1 maximal stoichiometry. PMID: 28561851
  2. This implies that a majority of the acidic residues within OPN must be engaged in calcium interaction under physiological conditions. PMID: 24928493
  3. Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. PMID: 24961487
  4. Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells. PMID: 25316140
  5. osteopontin (OPN) can serve as a process-directing agent for the intrafibrillar mineralization of collagen. PMID: 24140612
  6. Results suggest a mechanism of the interaction of UO2(2+) with bone metabolism and a new role for osteopontin (OPN) as a metal transporter. PMID: 24327307
  7. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation. PMID: 24019509
  8. OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. PMID: 22879891
  9. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine milk osteopontin (bmOPN) with post-translational modifications (PTMs) compared to human Escherichia-coli-derived recombinant OPN without PTMs. PMID: 19854170
  10. topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein PMID: 15004024
  11. the bovine osteopontin gene has two functionally distinct clusters of haplotypes within the QTL region on chromosome 6 PMID: 15867146
  12. The relationship between polymorphisms in SPP1 and several factors affecting growth and development in beef cattle was studied. PMID: 17179534
  13. findings show significant association of osteoponitin variants with milk composition traits PMID: 17517737
  14. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII. PMID: 17615552
  15. The binding of bovine seminal proteins A1, A2, and 30 kDa and osteopontin to spermatozoa and the effects of oviductal fluid on protein binding are reported. PMID: 18207674
  16. Results of this study confirm for the first time, the expression of Opn in the ileum and ileocecal lymph node of cattle with paratuberculosis. PMID: 18620757
  17. Opn may be a key regulator against Mycobacterium avium ssp. paratuberculosis infection PMID: 18650284
  18. The role of OPN phosphate groups in adsorption to, incorporation into and inhibition of calcium oxalate monohydrate crystals was studied by comparing OPN isoforms differing in phosphorylation. PMID: 18703867
  19. SPP1 (also known as osteopontin) has a significant role in the modulation of milk protein gene expression. PMID: 19392827
  20. OPN may act as a carrier protein for LF in milk, and modulate the potent antimicrobial and immunostimulatory activities of the LF protein. PMID: 19477017
  21. the present study is the first to demonstrate functionally that SPP1 directly binds specific integrins to promote trophectoderm cell migration and attachment to luminal epithelium that may be critical to conceptus elongation and implantation. PMID: 19571258
  22. Polymorphisms in the genomic sequence of SPP1 can influence the number of somatic cells in milk and, potentially, influence susceptibility to mastitis. PMID: 19765294

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Subcellular Location
Secreted.
Protein Families
Osteopontin family
Tissue Specificity
Bone, inner ear, kidney, uterus, lung, brain, epidermis.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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