Recombinant Bradyrhizobium sp. Formamidase(amiF)

In Stock
Code CSB-EP397462BPF
Product Type Recombinant Protein
Size US$2466
Uniprot No. A4Z3G9
Relevance Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP397462BPF could indicate that this peptide derived from E.coli-expressed Bradyrhizobium sp. (strain ORS278) amiF.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP397462BPF could indicate that this peptide derived from E.coli-expressed Bradyrhizobium sp. (strain ORS278) amiF.
Storage Buffer Tris-based buffer,50% glycerol
Alias Formamide amidohydrolase
Species Bradyrhizobium sp. (strain ORS278)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MNGLGGLNKSEHGVVIGLVQLQLPVVVTKEDLAKQTEKIVWMVGKARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDGPEVAAFKQACIDNKIWGCFSIMEYNPDGNPYNSGLIIDSNGEIKLYYRKLHPWIPVEPWEPGDLGIPVIEGPRGAKIALIICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCGSDGSFDSMGEGMIVNFDGSILAHGTTGRADEIITAEVRPDLVREARIGWGVENNIYQLWHRGYVAVKGGAMDCPYTFMHDMVAGTYRLPWEDQVKITDGTSCGFPAPTRVFGKMAKAAE
Research Area Others
Source E.coli
Gene Names amiF
Expression Region 1-337aa
Tag Info N-terminal 6xHis-SUMO-tagged
Mol. Weight 53.2kDa
Protein Description Full Length
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide.
Protein Families Carbon-nitrogen hydrolase superfamily, Aliphatic amidase family
Database Links

KEGG: bra:BRADO7112

STRING: 114615.BRADO7112

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