Recombinant Chicken Deleted in azoospermia-like(DAZL)

Code CSB-YP006514CH
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Source Yeast
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Code CSB-EP006514CH
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Source E.coli
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Code CSB-EP006514CH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006514CH
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Source Baculovirus
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Code CSB-MP006514CH
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names DAZL
Uniprot No. Q804A9
Alternative Names DAZLDeleted in azoospermia-like; DAZ-like protein
Species Gallus gallus (Chicken)
Expression Region 1-289
Target Protein Sequence MSANAEAQCG SISEDNTHSS TTCQGYVLPE GKIMPNTVFV GGIDIRMNEA EIRSYFEQYG TVKEVKIITD RTGVSKGYGF VSFLDNVDVQ KIVESQISVH GKRLKLGPAI RKQQNLCSYM QPRPLAFNPP APQFHSVWTN QNTETYVQPQ AVVSPLTQYV QTYAYSSPAV LIQQQVPVGY QPAYNYQAPP QWVPGEQRNY VMPPVYTSVN YHYSEDPEFI QTECAVPEPT QMSGNSPQKK SVDRSIQTVV SCLFNPENRL RNTFVSQEDY FRERRAHHFR KGRAVLKSV
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function RNA-binding protein, which probably plays a central role in gametogenesis in both males and females. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and promoting the translation of key transcripts (By similarity).
Gene References into Functions
  1. DAZL expression reveals formation and initial positioning of primordial germ cells in chickens. PMID: 26414995
  2. cDAZL expression is conserved in primordial germ cells and during germ-line differentiation until the adult stage, making them a valuable molecular marker for studies of PGC differentiation and germ-line development in chickens. PMID: 20537692
  3. cDAZL, CDH and CVH are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct PMID: 20332590
Subcellular Location Cytoplasm
Protein Families RRM DAZ family
Database Links

KEGG: gga:374054

STRING: 9031.ENSGALP00000018319

UniGene: Gga.5133

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