Recombinant Chicken GATA-binding factor 2(GATA2)

Code CSB-YP009275CH
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Source Yeast
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Code CSB-EP009275CH
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Source E.coli
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Code CSB-EP009275CH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009275CH
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Source Baculovirus
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Code CSB-MP009275CH
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names GATA2
Uniprot No. P23824
Alternative Names GATA2GATA-binding factor 2; GATA-2; Transcription factor NF-E1b
Species Gallus gallus (Chicken)
Expression Region 1-466
Target Protein Sequence MEVATDQPRW MTHHAVLNGQ HPESHHPGLA HNYMEPAQLL PPDEVDVFFN HLDSQGNPYY ANSAHARARV SYSQAHARLT GSQMCRPHLI HSPGIPWLDS SKAALSAHHH NPWTVNPFTK TPLHPSAAGA PGAISVYPGS STSSTASVSS LTPASHSGSH LFGFPPTPPK EVSPDPNSTS AASPSSSAGA RQEDKDSIKY QVSLSEGMKM ESASPLRSSL TSMGAQPSTH HPIPTYPSYV PAAHDYSSSL FHPGSFLGGP ASSFTPKPRS KARSCSEGRE CVNCGATATP LWRRDGTGHY LCNACGLYHK MNGQNRPLIK PKRRLSAARR AGTCCANCQT TTTTLWRRNA NGDPVCNACG LYYKLHNVNR PLTMKKEGIQ TRNRKMSNKS KKSKKGSECF EELSKCMQEK SSPFSAAALA SHMAPMGHLP PFSHSGHILP TPTPIHPSSS ISFGHPHPSS MVTAMG
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Transcriptional activator which probably serves as a general switch factor for cell-specific development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of genes.
Gene References into Functions
  1. Results provide new insights into the selective roles of Gata2 within the genetic model of V2 interneurons development and in particular on the hierarchy between V2-transcription factors PMID: 25369423
  2. Gata2 influences the site of primitive streak formation and its role is independent from, and upstream of, Vg1 and Wnt PMID: 23093427
  3. Within the rostral hindbrain, the Shh-activated homeodomain proteins Nkx2.2 and Nkx6.1 cooperate to induce the closely related zinc-finger transcription factors Gata2 and Gata3. (Gata2) PMID: 14973276
  4. data establish Gata2 and Gata3 as essential members of the transcription factor network controlling sympathetic neuron development PMID: 15329349
  5. At later stages, Gata2 expression became prominent in vestibular and cochlear nonsensory epithelia PMID: 17103399

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Subcellular Location Nucleus
Tissue Specificity Expressed in all developmental stages of erythroid cells but is additionally found in a limited subset of other tissues.
Database Links

KEGG: gga:416018

STRING: 9031.ENSGALP00000009491

UniGene: Gga.745

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