Recombinant Chicken GATA-binding factor 3 (GATA3)

Code CSB-YP009276CH
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Source Yeast
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Code CSB-EP009276CH
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Source E.coli
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Code CSB-EP009276CH-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009276CH
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Source Baculovirus
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Code CSB-MP009276CH
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GATA3
Uniprot No.
Alternative Names
GATA3; GATA-binding factor 3; GATA-3; Transcription factor NF-E1c
Species
Gallus gallus (Chicken)
Expression Region
1-444
Target Protein Sequence
MEVSTDQPRW VSHHHPAVLN GQHPDSHHPT LGHTYMDPTQ YPLAEEVDVL FNIDGQGNPV PPYYGNSVRA TVQRYPTAHH GSQVCRPPLL HGSLPWLDGS KALSSHHSAS PWNLSPFSKT SIHHSSPGPL SVYPPASSST LSAGHSSPHL FTFPPTPPKD VSPDPSISTP GSTGSTRQDE KECIKYQVSL ADTMKLESSH SRSSMASLGG ATSSAHHPIT TYPPYVPEYS SGLFPPSSLL GGSPTGFGCK SRPKARSSTE GRECVNCGAT STPLWRRDGT GHYLCNACGL YHKMNGQNRP LIKPKRRLSA ARRAGTSCAN CQTTTTTLWR RNANGDPVCN ACGLYYKLHN INRPLTMKKE GIQTRNRKMS SKSKKCKKVH DNLEDFPKSS SFNPAALSRH MSSISHISPF SHSSHMLTTP TPMHPPSSLS FGPHHPSSMV TAMG
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator which probably serves as a general switch factor for cell-specific development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of genes.
Gene References into Functions
  1. data establish Gata2 and Gata3 as essential members of the transcription factor network controlling sympathetic neuron development. PMID: 15329349
  2. GATA3 impacts WNT signaling in this region of the sensory macula. PMID: 19924793
  3. Gata3 was mainly expressed in the developing sensory epithelia, reflecting the importance of this factor in the sensory-neural development of the inner ear. PMID: 17103399
  4. During regeneration in the avian utricle, regionalized expression of GATA3 is maintained after severe hair cell lesions and after transplantation of the sensory epithelium onto a chemically defined substrate. PMID: 17154269
  5. Our results suggest that Gata3 may be specifically involved in the differentiation of auditory ganglion neurons during embryogenesis. PMID: 19673002

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed only in definitive (adult) erythrocytes within the red cell lineage, and is also abundantly expressed in T-lymphocytes and brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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