Recombinant Chicken Growth/differentiation factor 8 (MSTN)

Code CSB-YP015057CH
MSDS
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Source Yeast
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Code CSB-EP015057CH
MSDS
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Source E.coli
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Code CSB-EP015057CH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015057CH
MSDS
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Source Baculovirus
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Code CSB-MP015057CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MSTN
Uniprot No.
Alternative Names
MSTN; GDF8Growth/differentiation factor 8; GDF-8; Myostatin
Species
Gallus gallus (Chicken)
Expression Region
267-375
Target Protein Sequence
DFGL DCDEHSTESR CCRYPLTVDF EAFGWDWIIA PKRYKANYCS GECEFVFLQK YPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII YGKIPAMVVD RCGCS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts specifically as a negative regulator of skeletal muscle growth.
Gene References into Functions
  1. Studied three chicken lines to identify the polymorphism in the coding region of the myostatin gene. PMID: 27565871
  2. The c.234G>A substitution in the myostatin (MSTN) gene negatively regulates the expression of MSTN in the Bian chicken and that it may be used in marker-assisted selection to accelerate the chicken breeding process. PMID: 26513577
  3. These data imply that alternative splicing isoforms of MSTN could negatively regulate pro-myostatin processing in muscle cells and prevent MSTN-mediated inhibition of myogenesis in chickens, turkeys, and quail. PMID: 26354750
  4. The body weight gain and breast muscle yield of light chicks were improved by increasing dietary Methionine levels probably through alterations of MSTN transcription and phosphorylation of mTOR and FoxO4. PMID: 25437444
  5. Low daily feed intake did not alter the trend in myostatin (MSTN) mRNA expression levels in either line, but significantly changed the peak values. Myogenin (MYOG) mRNA expression varied by line and over time, with a delay in the Lean line. PMID: 24397509
  6. In conclusion, these studies suggested the decorin-mediated myostatin/Smad signaling pathway might be involved in the regulation of avian myoblast proliferation. PMID: 24570433
  7. results suggested that the E-boxes in the chicken MSTN promoter region are involved in the regulation of myostatin expression and the polymorphisms in E3 and E4 altered the expression of myostatin. PMID: 23670598
  8. Myostatin haplogroups had significant effect on chicken body weight at 28, 42, and 49 days of age in the PB-1 line and days 14 and 49 in the CB line. In the IWI line the myostatin gene was polymorphic with no significant association with growth traits. PMID: 23472013
  9. Association analysis showed that the G2283A were significantly associated with reproduction traits. Bian chickens of the GG genotype had a greater age at first egg than those of the GA and AA genotypes. PMID: 22870873
  10. G2283A mutation in the myostatin gene has the potential to be a genetic marker for body weight traits in the female Bian chicken. PMID: 20931358
  11. This study indicates that the autocrine action of myostatin is partially enhanced by IGF-I through increasing its expression. PMID: 19466527
  12. Myostatin (MSTN) is sensitive to nutrient supply in hatching chicks, and fasting reduced MSTN mRNA levels in muscles of older chickens. PMID: 14757186
  13. single nucleotide polymorphisms of the myostatin gene play an important role in controlling skeletal muscle growth and differentiation, and also may be involved in regulation of adipose growth PMID: 15382673
  14. myostatin was expressed in the ventral myotomal region of mature somites, confirming the importance of myostatin in skeletal muscle tissue formation during embryogenesis PMID: 16180589
  15. Myostatin is an important signalling molecule responsible for imposing quiescence upon myogenic precursors during embryonic and foetal development. PMID: 16425219
  16. These results suggest that deficiency of GDF-8 delays cell differentiation and causes great alterations in the cellular morphology of chicken embryonic myotubes. PMID: 16611734
  17. the ontogeny of myostatin expression coincided with myogenesis of chicken PMID: 16870219
  18. Role of endogenous myostatin in autocrine regulation of muscle growth. PMID: 17362950
  19. Two polymorphic sites in the first exon 1 of MSTN gene in 180 Wenling grass-chicken including 3 lines were studied by PCR-RFLP method,and its genetic effects on carcass traits were researched. PMID: 17548330
  20. GDF-8 is ubiquitously expressed in various tissues of chicken embryos including testis and ovary through the stage of embryogenesis. PMID: 17707668
  21. The present experiment was designed for studying the expression profiles of myostatin mRNA in the chicken liver, heart, brain, and intestine during their morphogenesis, using real-time PCR. PMID: 18037460
  22. results indicate that myostatin and peroxisome-proliferator-activator-gamma-coactivator-1alpha(PGC-1alpha) expression in skeletal muscle rapidly change in response acute cold PMID: 19427158
  23. These results suggest that the acute regulation of PGC-1alpha and myostatin gene expression in leg muscles is required for chicks to acquire cold tolerance up to 7 days of age. PMID: 19798921

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Subcellular Location
Secreted.
Protein Families
TGF-beta family
Database Links
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