Recombinant Chicken Osteopontin (SPP1)

Code CSB-YP022603CH
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP022603CH
MSDS
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Source E.coli
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Code CSB-EP022603CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022603CH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022603CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPP1
Uniprot No.
Alternative Names
SPP1; Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1
Species
Gallus gallus (Chicken)
Expression Region
17-264
Target Protein Sequence
WPVS KSRQHAISAS SEEKYDPRSH HTHRYHQDHV DSQSQEHLQQ TQNDLASLQQ THYSSEENAD VPEQPDFPDI PSKSQEAVDD DDDDDNDSND TDESDEVVTD FPTEAPVTPF NRGDNAGRGD SVAYGFRAKA HVVKASKLRK AARKLIEDDA TAEVGDSQLA GLWLPKESRE QDSRELAQHQ SVENDSRPRF DSPEVGGGDS KASAGVDSRE SLASRSAVDT SNQTLESAED AEDRHSIENN EVTR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Gene References into Functions
  1. results of this study indicate that SPP1 is novel in that its expression is down-regulated by estrogen in epithelial cells of the chicken oviduct and that it is up-regulated in chicken ovarian endometrioid tumor that could be used for monitoring effects of therapies for this disease in laying hens. PMID: 22588173
  2. fibroblast growth factor signaling directly activates osteopontin expression independent of chondrocytes differentiation PMID: 16253490
  3. Polymorphisms within the osteopontin gene (SPP1) are associated with 5-week body weight. PMID: 16734694
  4. OPN inhibited calcite growth in vitro at the crystallographic faces producing altered crystal morphology and circular growth step topography PMID: 18511297
  5. Matrix-mineral relationships in the eggshell at the ultrastructural level were studied using scanning and transmission electron microscopy, and the distribution of osteopontin is described. PMID: 18698131
  6. The effects of osteopontin and calbindin synthesis in the eggshell gland on the quality of egg shells in laying hens are reported. PMID: 19211537

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Subcellular Location
Secreted.
Protein Families
Osteopontin family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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