Recombinant Chicken Pituitary homeobox 2 (PITX2)

Code CSB-YP018043CH
MSDS
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Source Yeast
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Code CSB-EP018043CH
MSDS
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Source E.coli
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Code CSB-EP018043CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018043CH
MSDS
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Source Baculovirus
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Code CSB-MP018043CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
PITX2; PTX2; Pituitary homeobox 2; Homeobox protein PITX2; cPITX2; Paired-like homeodomain transcription factor 2
Species
Gallus gallus (Chicken)
Expression Region
1-333
Target Protein Sequence
MSCMKDPLSL ERLGAGNNKL CSSSPSSSSS SSSCHHQQPA LAMATALAPG QARSSLEAAK HRLEVHTISD TSSPEAAEKE KSQQGKSEDA GPEDPSKKKR QRRQRTHFTS QQLQELEATF QRNRYPDMST REEIAVWTNL TEARVRVWFK NRRAKWRKRE RNQQAELCKN GFGPQFNGLM QPYDDMYPGY SYNNWAAKGL TSASLSTKSF PFFNSMNVNP LSSQSMFSPP NSISSMSMSS SMVPSAVTGV PGSGLNSLNN LNNLSNPSLN SAVPTPACPY APPTPPYVYR DTCNSSLASL RLKAKQHSSF GYASVQNPAS NLSACQYPVD RPV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play an important role in development and maintenance of anterior structures. May play a role in determining left-right asymmetry and in vasculogenesis during avian embryogenesis.
Gene References into Functions
  1. Pitx2 is essential for axis formation, and that it acts as a direct regulator of cVg1 expression by binding to enhancers within neighbouring genes. PMID: 25496870
  2. Pitx2 pathway drives organ-specific arterial and lymphatic development in the intestine PMID: 25482882
  3. Downregulation of PITX2 and LEF1 genes may interfere with ventral body wall formation in Cd chick model causing omphalocele by disrupting somite myogenesis. PMID: 21259014
  4. Wnt/Lef1 signaling regulates epaxial myogenesis via Pitx2. PMID: 19850024
  5. Data suggest that the Pitx2c N-terminal domain competes with endogenous Pitx2c for binding to a protein interaction partner that is required for the activation of genes that direct asymmetric morphogenesis along the left-right axis. PMID: 19681163
  6. Mechanism in females that actively promotes the underlying L-R asymmetry initiated by Pitx2 and the degeneration of the right gonad, and a mechanism in males that allows it to be ignored or overridden. PMID: 17959721
  7. Pitx2 is together with Pax6 expressed in the entire domain of the Rathke's pouch. PMID: 18647663
  8. Pitx2 activity directs asymmetrical gonad morphogenesis by controlling mitotic spindle orientation of the developing gonad cortex and how, by modulating cyclinD1 expression during asymmetric ovarian development PMID: 18678914

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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