Recombinant Chicken Protein Wnt-2b (WNT2B)

Code CSB-YP857835CH
MSDS
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Source Yeast
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Code CSB-EP857835CH
MSDS
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Source E.coli
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Code CSB-EP857835CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857835CH
MSDS
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Source Baculovirus
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Code CSB-MP857835CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WNT2B
Uniprot No.
Alternative Names
WNT2B; Protein Wnt-2b
Species
Gallus gallus (Chicken)
Expression Region
21-385
Target Protein Sequence
ECGAFPRTAG AAPRICLPFV LILLALTPRA DSSWWYIGAL GARVICDNIP GLVNKQRQLC QRYPDIMQSV GEGAKEWIRE CQYQFRHHRW NCSTLDRDHT VFGRVMLRSS REAAFVYAIS SAGVVYAITR ACSQGELKAC GCDPLKRGRA KDERGEFDWG GCSDNINYGI RFAKAFVDAK EKKVKDARAL MNLHNNRCGR MAVKRFLKLE CKCHGVSGSC TLRTCWLAMS DFRKTGDYLQ KKYNGAIQVI MNQDGTGFTV ANKNFRKPTK TDLVYFENSP DYCVMDKSAG SLGTAGRVCN KVSRGTDGCE VMCCGRGYDT TRVTRVTKCE CKFHWCCAVR CKECEDTVDV HTCKAPKRAE WLDQT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Acts as an upstream regulator of FGF10 in the lateral plate mesoderm during limb initiation in the embryo.
Gene References into Functions
  1. Neural crest-derived TGFbs activate both Smad3 and Wnt2b signalling in the adjacent ectoderm to position the lens next to the retina. PMID: 21468017
  2. The anterior optic cup domain, as described in part by Wnt2b expression, is specified through the elaboration of a border within the optic neuroepithelium rather than through interactions with the surrounding environment. PMID: 21179237
  3. Wnt2b expression in the anterior rim of the developing retina is responsible for laminar structure of the retina layer. PMID: 12921742
  4. WNT2B regulates embryonic forelimb development via FGF signaling and WNT/b-catenin signaling PMID: 11290326
  5. Wnt2b maintains the naive state of marginal progenitor cells by attenuating the expression of both proneural and neurogenic genes PMID: 15901663
  6. The expression of Wnt2b or constitutively active beta-catenin inhibited retinal progenitor gene (RPG) expression and the differentiation of retinal neurons. PMID: 16854977

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
Detected at the marginal tip of the developing retina. Expressed in the intermediate and the lateral plate mesoderm in the presumptive chick forelimb region. Expressed in the eye, head ectoderm, prospective forelimb mesenchyme, lung bud, pharyngeal arches
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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