Code | CSB-EP006187DXU |
Size | US$388 |
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CUSABIO synthesized the recombinant gene by integrating the N-terminal 6xHis-SUMO tag sequence into the targeted gene encoding the 65-408aa of the Cricetulus griseus CTSD. The synthesized gene was subsequently cloned into an expression vector. After cloning, the expression vector was introduced into the E.coli for expression. The product was purified to obtain the recombinant Cricetulus griseus CTSD protein carrying N-terminal 6xHis-SUMO tag. The SDS-PAGE assayed the purity of this recombinant CTSD protein greater than 90%. This CTSD protein migrated along the gel to a band of about 50 kDa molecular weight.
Cathepsin D is a protein encoding by a gene named H671_3g9701 in chinese hamster or a gene named CTSD in human. Cathepsin D is an acid lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsin D can dissolve basement membrane in an acidic environment, degrade extracellular matrix and connective tissue, and is related to aggressive biological behavior. The specificity of cathepsin D is similar topepsin A, but it can not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Studies have confirmed that Cathepsin D plays an important role in tumorigenesis and development.
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