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Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5)

Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5)

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Code	CSB-YP317769CTL
Abbreviation	Recombinant Cryptococcus neoformans var. neoformans serotype D URA5 protein
MSDS	MSDS Datasheet
Size	$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

URA5

Uniprot No.

P0CS95

Research Area

others

Alternative Names

URA5; CNG03730; Orotate phosphoribosyltransferase; OPRT; OPRTase; EC 2.4.2.10

Species

Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)

Source

Yeast

Expression Region

1-225aa

Target Protein Sequence

MSSQALDSAKVAFIEAAIEHGVLLFGNFTLKSGRQSPYFFNAGLLYSSSLLSTTAQAYAKVLSSSRIPDFDVLFGPAYKGISLAAVSAVSLYQQTGKDIGYCYNRKEKKDHGEGGTMVGAPLKGRIVIIDDVLTSGKAIREAIDILKASPEAKLVGIVQLVDRQEKGQSGSGKSTVQEVEEEFGVPVEPIIGLDDIVKYLESSGKWEKELQEVRKYRAEYGVQRS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.4kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) URA5 protein in Yeast cells, a DNA fragment encoding the Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) URA5 protein (1-225aa) is inserted into a plasmid vector, which is then transferred to Yeast cells. After screening positive cells, they are cultured and induced to generate the URA5 protein. A N-terminal 10xHis tag and C-terminal Myc tag is attached to the protein. Cells are lysed to collect the recombinant Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) URA5 protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the obtained recombinant Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) URA5 protein is over 90%.

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Target Background

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).

Protein Families

Purine/pyrimidine phosphoribosyltransferase family, PyrE subfamily

Database Links

KEGG: cne:CNG03730

STRING: 214684.XP_572079.1

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