Recombinant Cynodon dactylon Major pollen allergen Cyn d 1 (CYND1)

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Code CSB-EP516895EQB
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
CYND1
Uniprot No.
Research Area
allergen
Alternative Names
CYND1; Major pollen allergen Cyn d 1; allergen Cyn d 1
Species
Cynodon dactylon (Bermuda grass) (Panicum dactylon)
Source
E.coli
Expression Region
1-246aa
Target Protein Sequence
AIGDKPGPNITATYGSKWLEARATFYGSNPRGAAPDDHGGACGYKDVDKPPFDGMTACGNEPIFKDGLGCRACYEIKCKEPVECSGEPVLVKITDKNYEHIAAYHFDLSGKAFGAMAKKGQEDKLRKAGELTLQFRRVKCKYPSGTKITFHIEKGSNDHYLALLVKYAAGDGNIVAVDIKPRDSDEFIPMKSSWGAIWRIDPKKPLKGPFSIRLTSEGGAHLVQDDVIPANWKPDTVYTSKLQFGA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
31.9kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Cynodon dactylon CYND1 was expressed with the amino acid range of 1-246. The expected molecular weight for the CYND1 protein is calculated to be 31.9 kDa. This CYND1 recombinant protein is manufactured in e.coli. Fusion of the N-terminal 10xHis tag and C-terminal Myc tag into the CYND1 encoding gene fragment was conducted, allowing for easier detection and purification of the CYND1 protein in subsequent stages.

Cynodon dactylon Major Pollen Allergen CYND1 is a significant allergenic protein found in the pollen of Bermuda grass (Cynodon dactylon). Its primary function is triggering allergic reactions in individuals sensitive to grass pollen. In allergy research and immunology, CYND1 is a focus for understanding grass pollen-induced allergies. Research areas include identifying the role of CYND1 in allergic responses and developing diagnostic tools and therapies for pollen allergies. The importance of CYND1 lies in its contribution to allergic sensitization and efforts to mitigate the impact of grass pollen allergies on affected individuals.

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Target Background

Subcellular Location
Secreted.
Protein Families
Expansin family, Expansin B subfamily
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