Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
cops2; csn2; zgc:86624COP9 signalosome complex subunit 2; Signalosome subunit 2
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-443aa
Target Protein Sequence
MSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFKTNTKLGKLYLEREEFGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIREHIEELLRNIRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTINGRIDQVNQLLELDHQKRGGARYTALDKWTNQLNSLNQAIVSKLA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Zebrafish cops2 contains amino acids 1-443. The expected molecular weight for the cops2 protein is calculated to be 57.1 kDa. This cops2 protein is produced using e.coli expression system. Fusion of the N-terminal 10xHis tag into the cops2 encoding gene fragment was conducted, allowing for easier detection and purification of the cops2 protein in subsequent stages.
Danio rerio COP9 signalosome complex subunit 2 (COPS2) is a component of the COP9 signalosome, a multi-protein complex involved in regulating protein degradation and signaling pathways. COPS2 mainly participates in the ubiquitin-proteasome system, influencing protein stability and cellular processes. In developmental biology, COPS2 is implicated in embryogenesis and organogenesis. In cancer research, COPS2's role in proteasomal regulation links it to tumorigenesis. Investigating COPS2 provides insights into cellular homeostasis, developmental processes, and disease mechanisms. Additionally, COPS2 may serve as a potential target for therapeutic interventions in cancer.
