Recombinant Danio rerio POU domain, class 5, transcription factor 1 (pou5f1)

1. Pou2 is essential for endoderm formation. Embryos devoid of maternal and zygotic pou2 lack endodermal precursors. PMID: 14723850
2. Western blot analysis were conducted on developing hatchlings with Oct4 antibody and at 13.6 and 21.6mugL(-1)dose,it showed over expression elucidating stimulatory role of nanosilver. In silico analysis depicted a firm interaction of nanosilver with Oct4 revealing their key role in growth stimulation of developing embryos. PMID: 29778081
3. The authors show, at single-cell resolution in vivo, that Pou5f3 complexes with Nanog to pattern mesendoderm differentiation at the blastula stage. PMID: 27684073
4. Data suggest that, in developing gastrula, Znfl1 controls developmental gene expression of Hoxb1b in embryonic posterior neuroectoderm by acting upstream of Pou5f3 and Sall4; these proteins appear to be involved in neurogenesis. (Znfl1 = zinc finger-like gene 1; Hoxb1b = homeobox B1b protein; Pou5f3 = POU domain class 5 transcription factor 3; Sall4 = spalt-like transcription factor 4) PMID: 28623229
5. Regulation of mych by Pou5f1 appears to be direct transcriptional activation. PMID: 24643012
6. The posttranslational modification by phosphorylation opens the possibility that Pou5f1 may be subject to temporal or region specific modulation of its activity or stability by embryonic signaling mechanisms. PMID: 24130110
7. discuss mechanistic implications of simultaneous activation of transcriptional targets by ubiquitous, like Pou5f1, and region-specific inducers, emerging as a common regulatory motif in early development PMID: 24211655
8. maternal Nanog, Pou5f1 and SoxB1 are required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression PMID: 24056933
9. thses data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control. PMID: 23950494
10. The defects due to HEP induction were rescued by introducing wild-type pou2 mRNA before the heat treatments. PMID: 22921661
11. Vox plays a key role downstream of BMP signals in regulating the capacity of Nodal to induce endoderm versus mesoderm by modulating the activity of the Casanova/Pou2 regulatory system. PMID: 23364327
12. Pou2 functions in multiple aspects of vertebrate development, especially in the binary decision of the mesendoderm to mesoderm and endoderm in different ways depending on the developmental stage. PMID: 22913532
13. show that Pou5f1 binds to phylogenetically conserved Oct/Pou5f1 sites in the vox promoter, both in vivo and in vitro PMID: 21621531
14. The temporospatial structure of the zebrafish Pou5f1 target networks may explain aspects of the evolution of the mammalian stem cell networks. PMID: 20212526
15. Data show that embryos genetically depleted of both maternal and zygotic pou2 function exhibit extreme dorsoventral patterning defects and, independently, a blastoderm-specific arrest of epiboly. PMID: 16775002
16. Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defects of Pou5f1/Oct4 deficient MZspg zebrafish embryos. PMID: 18215655
17. These data provide a scheme wherein pou2/pou5f1 expression in zebrafish embryos is regulated by both an autoregulatory loop and repression by RA emanating from the posterior mesoderm. PMID: 18407549

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KEGG: dre:30333

STRING: 7955.ENSDARP00000065816

UniGene: Dr.258