POU5F1

POU5F1 is also known as Oct3, Oct4, Otf3 or NF-A3. It is encoded by the POU5F1 gene, which belongs to POU transcription factor family, class-5 subfamily.

POU5F1 gene and located on the human chromosome 6p21.3. It has multiple transcription initiation sites, leading to a diversity of POU5F1 proteins. There are four protein subtypes (Oct4A, oct4b-190, oct4b-265 and oct4b-164).

Oct4 is mainly expressed in embryonic stem cells, embryonic germ cells and embryonic/germ cell tumors, but is less expressed in adult tissues. In embryonic germ cells, Oct4 expression is down-regulated as cells differentiate.

Oct4 consists of three domains: N-transcription domain, POU binding domain and C-transcription domain. POU binding domain is a conserved DNA binding domain that binds to DNA containing an octamer motif to regulate transcription of downstream target genes.

Oct4 participates in the regulation of several signaling pathways, including transforming growth factor (TGF) beta pathway, fibroblast growth factor (FGF) pathway, phosatidylinositol-3-kinase (PI3K) pathway, Wnt pathway, etc.

POU5F1 regulates the expression of target genes by combining with octamer motif (5'-ATTTGCAT-3') in promoter or enhancer regions

POU5F1 is critical for early embryogenesis and for embryonic stem cell pluripotency. In addition, POU5F1 may be involved in the occurrence and development of malignant tumors.

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CSB-MA018403A0m (Oct4 Monoclonal Antibody) is a product of CUSABIO. The product can be used in human, rat and mouse. It has been validated in various applications, including ELISA, WB, IHC, IF and FC.